Establishment of A New HBV Cell Culture Model by Covalently Closed Circular DNA Direct Transfect

被引：1
作者：
Zhao, Zhonghua [1 ]
Tang, Yuwei [1 ]
Wei, Qinglv [1 ]
Zhang, Huatang [1 ]
机构：
[1] Chongqing Acad Sci & Technol, Chongqing, Peoples R China
来源：
2020 ASIA CONFERENCE ON GEOLOGICAL RESEARCH AND ENVIRONMENTAL TECHNOLOGY | 2021年 / 632卷
基金：
中国国家自然科学基金;
关键词：
HEPATITIS-B-VIRUS; INFECTION;
D O I：
10.1088/1755-1315/632/3/032023
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Hepatitis B virus (HBV) infection is a public health problem in China and worldwide. Covalently closed circular DNA (cccDNA) is the template for HBV replication and exists stably in hepatocytes, which is the main factor for persistent HBV infection and the key target for the cure of HBV infection. The establishment of a suitable cell model is helpful to research the HBV pathogenesis and antiviral drug screening. We have established a new cell culture model of HBV infection by direct transfect of cccDNA, which provides a practical model for further research of HBV virology and antiviral drug development.
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页数：7
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共 24 条
  • [1] The Role of cccDNA in HBV Maintenance
    Allweiss, Lena
    Dandri, Maura
    [J]. VIRUSES-BASEL, 2017, 9 (06):
  • [2] BEASLEY RP, 1981, LANCET, V2, P1129
  • [3] A new hepatitis B virus e antigen-negative strain gene used as a reference sequence in an animal model
    Cao, Min
    Zhao, Zhonghua
    Tang, Yuwei
    Wei, Qinglv
    Wang, Lei
    Xiang, Qin
    Zhang, Yunmei
    Zhang, Huatang
    Lai, Guoqi
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 496 (02) : 502 - 507
  • [4] A Novel Quantitative Microarray Antibody Capture Assay Identifies an Extremely High Hepatitis Delta Virus Prevalence Among Hepatitis B Virus-Infected Mongolians
    Chen, Xiaohua
    Oidovsambuu, Odgerel
    Liu, Ping
    Grosely, Rosslyn
    Elazar, Menashe
    Winn, Virginia D.
    Fram, Benjamin
    Boa, Zhang
    Dai, Hongjie
    Dashtseren, Bekhbold
    Yagaanbuyant, Dahgwahdorj
    Genden, Zulkhuu
    Dashdorj, Naranbaatar
    Bungert, Andreas
    Dashdorj, Naranjargal
    Glenn, Jeffrey S.
    [J]. HEPATOLOGY, 2017, 66 (06) : 1739 - 1749
  • [5] Modeling hepatitis B virus infection, immunopathology and therapy in mice
    Cheng, Liang
    Li, Feng
    Bility, Moses T.
    Murphy, Christopher M.
    Su, Lishan
    [J]. ANTIVIRAL RESEARCH, 2015, 121 : 1 - 8
  • [6] GANEM D, 1987, ANNU REV BIOCHEM, V56, P651, DOI 10.1146/annurev.biochem.56.1.651
  • [7] Infection of a human hepatoma cell line by hepatitis B virus
    Gripon, P
    Rumin, S
    Urban, S
    Le Seyec, J
    Glaise, D
    Cannie, I
    Guyomard, C
    Lucas, J
    Trepo, C
    Guguen-Guillouzo, C
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) : 15655 - 15660
  • [8] HEPATITIS-B VIRUS-INFECTION OF ADULT HUMAN HEPATOCYTES CULTURED IN THE PRESENCE OF DIMETHYL-SULFOXIDE
    GRIPON, P
    DIOT, C
    THEZE, N
    FOUREL, I
    LOREAL, O
    BRECHOT, C
    GUGUENGUILLOUZO, C
    [J]. JOURNAL OF VIROLOGY, 1988, 62 (11) : 4136 - 4143
  • [9] Generation of Covalently Closed Circular DNA of Hepatitis B Viruses via Intracellular Recycling Is Regulated in a Virus Specific Manner
    Koeck, Josef
    Roesler, Christine
    Zhang, Jing-Jing
    Blum, Hubert E.
    Nassal, Michael
    Thoma, Christian
    [J]. PLOS PATHOGENS, 2010, 6 (09)
  • [10] Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: A novel system for screening potential inhibitors of HBV replication
    Ladner, SK
    Otto, MJ
    Barker, CS
    Zaifert, K
    Wang, GH
    Guo, JT
    Seeger, C
    King, RW
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (08) : 1715 - 1720
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