Triple helices formed at oligopyrimidine•oligopurine sequences with base pair inversions:: effect of a triplex-specific ligand on stability and selectivity

被引：28
作者：
Kukreti, S
Sun, JS
Loakes, D
Brown, DM
Nguyen, CH
Bisagni, E
Garestier, T
Helene, C
机构：
[1] Museum Natl Hist Nat, Biophys Lab, CNRS, URA 481,INSERM,U201, F-75231 Paris 05, France
[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[3] Inst Curie, CNRS, UMR 176, Organ Synth Lab, F-91405 Orsay, France
来源：
NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 09期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1093/nar/26.9.2179
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligonucleotide-directed triple helix formation is mostly restricted to oligopyrimidine oligopurine sequences of double helical DNA. An interruption of one or two pyrimidines in the oligopurine target strand leads to a strong tripler destabilisation. We have investigated the effect of nucleotide analogues introduced in the third strand at the site opposite the base pair inversion(s). We show that a 3-nitropyrrole derivative (M) discriminates G.C from C.G, A.T and T.A in the presence of a tripler-specific ligand (a benzo[e]pyridoindole derivative, BePI). N6-methoxy-2,6-diaminopurine (K) binds to an A.T base pair better than a T.A, G.C or C.G base pair. Some discrimination is still observed in the presence of BePI and tripler stability is markedly increased. These findings should help in designing BePI-oligonucleotide conjugates to extend the range of DNA sequences available for tripler formation.
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页码：2179 / 2183
页数：5
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