Label-Free in Situ SERS Imaging of Biofilms

被引:97
作者
Ivleva, Natalia P. [1 ]
Wagner, Michael [2 ]
Szkola, Agathe
Horn, Harald [2 ]
Niessner, Reinhard
Haisch, Christoph
机构
[1] Tech Univ Munich, Chair Analyt Chem, Inst Hydrochem, D-81377 Munich, Germany
[2] Tech Univ Munich, Inst Water Qual Control, D-85478 Garching, Germany
关键词
SURFACE-ENHANCED RAMAN; SCATTERING SERS; BACTERIAL-CELLS; SPECTROSCOPIC CHARACTERIZATION; CHEMICAL-CHARACTERIZATION; RELEVANT MICROORGANISMS; SILVER NANOPARTICLES; GOLD NANOPARTICLES; COLLOIDAL SILVER; ANALYTICAL TOOL;
D O I
10.1021/jp102466c
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Surface-enhanced Raman scattering (SERS) is a promising technique for the chemical characterization of biological systems. It yields highly informative spectra, can be applied directly in aqueous environment, and has high sensitivity in comparison with normal Raman spectroscopy Moreover, SERS imaging can provide chemical information with spatial resolution in the micrometer range (chemical imaging). In this paper, we report for the first time on the application of SERS for in situ, label-free imaging of biofilms and demonstrate the suitability of this technique for the characterization of the complex biomatrix. Biofilms, being communities of microorganisms embedded in a matrix of extracellular polymeric substances (EPS), represent the predominant mode of microbial life Knowledge of the chemical composition and the structure of the biofilm matrix is Important in different fields, e g, medicine, biology, and industrial processes We used colloidal silver nanoparticles for the in situ SERS analysis. Good SERS measurement reproducibility, along with a significant enhancement of Raman signals by SERS (> 10(4)) and highly informative SERS signature, enables rapid SERS imaging (1 s for a single spectrum) of the biofilm matrix Altogether, this work illustrates the potential of SERS for biofilm analysis, including the detection of different constituents and the determination of their distribution in a biofilm even at low biomass concentration.
引用
收藏
页码:10184 / 10194
页数:11
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