Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry

被引：49
作者：
Qi, Cai [2 ,3 ,5 ]
Tian, Xin-Sheng [1 ,3 ]
Chen, She [2 ,3 ]
Yan, Jing-Hua [1 ]
Cao, Zhen [4 ]
Tian, Ke-Gong [4 ]
Gao, George F. [1 ]
Jin, Gang [2 ]
机构：
[1] Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China
[2] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China
[3] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[4] China Anim Dis Control Ctr, Beijing 100094, Peoples R China
[5] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 06期
关键词：
Avian influenza virus; Biosensor; Ellipsometry; Scanning near-field optical microscopy; OFF-NULL ELLIPSOMETRY; PROTEIN; SURFACE; BINDING;
D O I：
10.1016/j.bios.2009.10.030
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic toot for avian influenza virus detection. (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：1530 / 1534
页数：5
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