Super-resolution lensless microscopy using multi-wavelength multiplexing

We report a multi-wavelength multiplexed setup and associated super-resolution reconstruction method in lensless microscopy, which can generate high-resolution reconstructions from undersampled raw measurements captured at multiple wavelengths. The reconstruction result of the Benchmark Quantitative Phase Microscopy Target (QPTTM) demonstrates the resolution enhancement quantitatively, which achieves a half-pitch lateral resolution of 691 nm across a large field of view (similar to 29.85 mm(2)), surpassing 2.41 times of the theoretical Nyquist-Shannon sampling resolution limit imposed by the pixel-size of the sensor (1.67 mu m). Compared with other super-resolution methods such as lateral or axial shift-based device and illumination source rotation design, multi-wavelength multiplexed method avoids the need for shifting/rotating mechanical components. This multi-wavelength multiplexed super-resolution method would benefit the research and development of a more stable lensless microscopy system.