Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins

被引:22
作者
Qiao, Meiling [1 ]
Tu, Maolin [2 ]
Chen, Hui [1 ]
Mao, Fengjiao [1 ]
Yu, Cuiping [1 ]
Du, Ming [1 ]
机构
[1] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, Dalian 116034, Peoples R China
[2] Harbin Inst Technol, Dept Food Sci & Engn, Harbin 150090, Heilongjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Mytilus edulis; enzymatic hydrolysis; UPLC-Q-TOF-MS/MS; molecular docking; anticoagulant activity; ACE INHIBITORY PEPTIDE; MOLECULAR DOCKING; BLUE MUSSEL; BIOACTIVE PEPTIDES; ANTIOXIDANT ACTIVITY; MASS-SPECTROMETRY; THROMBIN; DIGESTION; PURIFICATION; COAGULATION;
D O I
10.3390/ijms19072100
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mytilus edulis is a typical marine bivalve mollusk. Many kinds of bioactive components with nutritional and pharmaceutical activities in Mytilus edulis were reported. In this study, eight different parts of Mytilus edulis tissues, i.e., the foot, byssus, pedal retractor muscle, mantle, gill, adductor muscle, viscera, and other parts, were separated and the proteins from these tissues were prepared. A total of 277 unique peptides from the hydrolysates of different proteins were identified by UPLC-Q-TOF-MS/MS, and the molecular weight distribution of the peptides in different tissues was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The bioactivity of the peptides was predicted through the Peptide Ranker database and molecular docking. Moreover, the peptides from the adductor muscle were chosen to do the active validation of anticoagulant activity. The active mechanism of three peptides from the adductor muscle, VQQELEDAEERADSAEGSLQK, RMEADIAAMQSDLDDALNGQR, and AAFLLGVNSNDLLK, were analyzed by Discovery Studio 2017, which also explained the anticoagulant activity of the hydrolysates of proteins from adductor muscle. This study optimized a screening and identification method of bioactive peptides from enzymatic hydrolysates of different tissues in Mytilus edulis.
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页数:12
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