Enhanced growth by ectopic expression of growth hormone releasing hormone using an injectable myogenic vector

被引：51

作者：

Draghia-Akli, R ^{[1
]}

Li, XY ^{[1
]}

Schwartz, RJ ^{[1
]}

机构：

[1] Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA

来源：

NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 12期

关键词：

gene therapy; growth hormone; DNA vaccine; skeletal alpha-actin;

D O I：

10.1038/nbt1197-1285

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ectopic expression of a truncated growth hormone-releasing hormone (GHRH) from muscle tissues by a myogenic plasmid DNA vector directed by the skeletal alpha-actin promoter, pSK-GHRH, results in growth hormone (GH) secretion. Skeletal muscle-secreted GHRH is biologically active. The application of conditioned media harvested from pSK-GHRH transfected muscle cells to cultured pig primary anterior pituitary cells elicits GH release. A single intramuscular injection of 100 mu g pSK-GHRH DNA elevates serum GH levels threefold to fourfold for up to 2 weeks, enhancing liver IGF-1 gene expression and increasing body weight approximately 10%.

引用

页码：1285 / 1289

页数：5

共 45 条

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