Mismatch repair gene mutations in renal cell carcinoma

被引:22
作者
Leach, FS [1 ]
Koh, M [1 ]
Sharma, K [1 ]
McWilliams, G [1 ]
Talifero-Smith, L [1 ]
Codd, A [1 ]
Olea, R [1 ]
Elbahloul, O [1 ]
机构
[1] NCI, Urol Oncol Branch, NIH, Bethesda, MD 20892 USA
关键词
mismatch repair; hMLH; 1; renal cell carcinoma; microsatellite instability;
D O I
10.4161/cbt.1.5.171
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We investigated the spectrum and genetic basis for mismatch repair (MMR) deficiency in renal cell carcinoma (RCC) by examining expression of four MMR genes important for hereditary and sporadic carcinogenesis. MMR deficiency was assessed using microsatellite instability (MSI) and genetic analyses of 25 cell lines derived from renal tumors. MMR gene alterations were detected using reverse transcription of RNA coupled with polymerase chain reaction (RT-PCR) and DNA sequencing. Three RCC lines with undetectable MLH1 were identified and investigated for MSI and inactivating mutations in the hMLH1 MMR gene. Genetic instability and hMLH1 mutations were identified in two RCC lines and their corresponding tumors. Genetic alterations affecting expression were limited to MLHI since other MMR proteins (MSH2, MSH6 and PMS2) were detectable in our RCC lines. Complete inactivation of MMR is apparently uncommon in RCC and occurs predominantly through inactivating mutations in the hMLH1 gene.
引用
收藏
页码:530 / 536
页数:7
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