Multiplex PCR for the identification of white Tuber species

被引：1
|
作者：
Amicucci, A
Guidi, C
Zambonelli, A
Potenza, L
Stocchi, V
机构：
[1] Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, PS, Italy
[2] Univ Bologna, Dipartimento Protez & Valorizzaz Agroalimentare, I-40126 Bologna, Italy
来源：
FEMS MICROBIOLOGY LETTERS | 2000年 / 189卷 / 02期
关键词：
Tuber; molecular identification; multiplex polymerase chain reaction; Sphaerosporella brunnea;
D O I：
10.1016/S0378-1097(00)00296-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Species-specific primers selected from the internal transcribed spacer region sequence were used to set up a multiplex polymerase chain reaction (PCR) able to simultaneously identify the white truffle species Tuber magnatum, Tuber borchii, Tuber maculatum and Tuber puberulum. Furthermore, a primer specific for the competitive fungus Sphaerosporella brunnea was designed and added to the multiplex PCR set, allowing the detection of the Tuber species and the contaminant fungus in a one-step reaction. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：265 / 269
页数：5
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