HOW Is Required for Stem Cell Maintenance in the Drosophila Testis and for the Onset of Transit-Amplifying Divisions

被引：37

作者：

Monk, Adrian C. ^{[1
,2
]}

Siddall, Nicole A. ^{[1
,2
]}

Volk, Talila ^{[3
]}

Fraser, Barbara ^{[2
,4
]}

Quinn, Leonie M. ^{[1
]}

McLaughlin, Eileen A. ^{[2
,4
]}

Hime, Gary R. ^{[1
,2
]}

机构：

[1] Univ Melbourne, Dept Anat & Cell Biol, Melbourne, Vic 3010, Australia

[2] ARC Ctr Excellence Biotechnol & Dev, Callaghan, NSW 2308, Australia

[3] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

[4] Univ Newcastle, Sch Environm & Life Sci, Callaghan, NSW 2308, Australia

来源：

CELL STEM CELL | 2010年 / 6卷 / 04期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

RNA-BINDING PROTEIN; DEVELOPMENTAL REGULATORS; SELF-RENEWAL; ISOFORMS; GERM-CELL; CYCLIN-A; DIFFERENTIATION; SPERMATOGENESIS; PROLIFERATION; DEGRADATION;

D O I：

10.1016/j.stem.2010.02.016

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The mechanisms by which germline stem cells (GSCs) in the Drosophila testis undergo asymmetric division to regenerate a stem cell as well as a daughter (gonialblast) that will only undergo a further four mitotic divisions prior to entering premeiotic S phase and differentiating into a cyst of spermatocytes are not fully resolved. Here we demonstrate that the HOW RNA-binding protein is required for maintenance of CycB and therefore mitotic progression in GSCs and gonialblasts as well as determining the timing of the spermatogonial divisions. HOW is normally expressed in a complementary pattern to Barn in the germline and barn mRNA is bound by HOW in vivo. Ectopic expression of the HOW(L) isoform is associated with a delay in accumulation of Barn to the level required for differentiation, resulting in extra mitotic divisions. Spatiotemporal regulation of HOW expression is therefore required to specify the four spermatogonial transit-amplifying divisions.

引用

页码：348 / 360

页数：13