Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles

被引：38

作者：

Boerneke, Mark A. ^{[1
]}

Dibrov, Sergey M. ^{[1
]}

Hermann, Thomas ^{[1
,2
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Ctr Drug Discovery Innovat, 9500 Gilman Dr, La Jolla, CA 92093 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 12期

基金：

美国国家卫生研究院;

关键词：

nanostructures; RNA structures; RNA switch; self-assembly; X-ray diffraction; ARCHITECTURE; SWITCHES; COMPLEX; SQUARE; DOMAIN;

D O I：

10.1002/anie.201600233

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

RNA nanotechnology uses RNA structural motifs to build nanosized architectures that assemble through selective base-pair interactions. Herein, we report the crystal-structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6 angstrom resolution reveals the so-far smallest circularly closed nanoobject made entirely of double-stranded RNA. The assembly of the nanotriangle architecture involved RNA corner motifs that were derived from ligand-responsive RNA switches, which offer the opportunity to control self-assembly and dissociation.

引用

页码：4097 / 4100

页数：4

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