Cloning and characterization of bovine stearoyl CoA desaturasel cDNA from adipose tissues

被引：39

作者：

Chung, M

Ha, S

Jeong, S

Bok, J

Cho, K

Baik, M

Choi, Y ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Sch Agr Biotechnol, Dept Anim Sci & Technol, Suweon 441744, South Korea

[2] Univ Saskatchewan, Coll Med, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

[3] Chonnam Natl Univ, Dept Genet Engn, Kwangju 500757, South Korea

[4] Chonnam Natl Univ, Inst Biotechnol, Kwangju 500757, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 07期

关键词：

stearoyl CoA desaturase; cDNA; mRNA expression; bovine adipose tissues;

D O I：

10.1271/bbb.64.1526

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two overlapping cDNA clones have been isolated from bovine adipose tissue by the reverse-transcription-polymerase chain reaction (RT-PCR) method. The combined sequence of the two clones was 1039 bp in length and encoded 345 amino acids. The deduced amino acid sequence of the clones showed 96.5% similarity to that of sheep SCD and more than 88% similarities to other mammalian SCD1s, indicating that the clones are the cDNAs for the bovine SCD1. The transcript size of the bovine SCD1 was about 4.9 kb, the message was detected in the bovine adipose tissues but not in the liver. Female cattle expressed threefold higher levels of SCD1 mRNA than male animals.

引用

页码：1526 / 1530

页数：5

共 20 条

[1] STEAROYL COENZYME-A DESATURASE ENZYME-ACTIVITY AND MESSENGER-RNA LEVELS ARE NOT DIFFERENT IN SUBCUTANEOUS ADIPOSE-TISSUE FROM ANGUS AND AMERICAN WAGYU STEERS [J].