Calcium Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells

被引:3
|
作者
Ji, Lina [1 ]
Chauhan, Abha [1 ]
Chauhan, Ved [1 ]
机构
[1] New York State Inst Basic Res Dev Disabil, Dept Neurochem, Staten Isl, NY 10314 USA
关键词
Calcium; Gelsolin; Mitogen-activated protein kinases; Protein kinase C; PROTEIN-KINASE-C; AMYLOID BETA-PROTEIN; ALZHEIMERS-DISEASE BRAIN; HYDROGEN-PEROXIDE; CASPASE-CLEAVAGE; APOPTOSIS; PATHOLOGY; COMPLEX; MICE; IDENTIFICATION;
D O I
10.1007/s11064-010-0157-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gelsolin plays an important role in the regulation of amyloid beta-protein fibrillogenesis. We report here that calcium ionophore A23187 induces the expression of cytoplasmic gelsolin (c-gelsolin), and that protein kinase C (PKC) is involved in the up-regulation of c-gelsolin. In the presence of calcium, both SH-SY5Y and HEK-293 cells upon treatment with A23187 showed an increase in c-gelsolin expression in a concentration-dependent manner. Calcium-mediated up-regulation of c-gelsolin was inhibited by cycloheximide (a general inhibitor of protein synthesis). When cells were pretreated with staurosporine (an inhibitor of a variety of protein kinases including PKC), the up-regulation of c-gelsolin induced by A23187 was inhibited. Calphostin C, an inhibitor of PKC, blocked the up-regulation of c-gelsolin induced by A23187, while inhibitors of mitogen-activated protein kinases had no effect on c-gelsolin expression. In addition, phorbol-12-myristate-13-acetate, an activator of PKC, up-regulated c-gelsolin expression. These results suggest that calcium mediates up-regulation of c-gelsolin in a PKC-dependent manner.
引用
收藏
页码:1075 / 1082
页数:8
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