Calcium Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells

被引：3

作者：

Ji, Lina ^{[1
]}

Chauhan, Abha ^{[1
]}

Chauhan, Ved ^{[1
]}

机构：

[1] New York State Inst Basic Res Dev Disabil, Dept Neurochem, Staten Isl, NY 10314 USA

来源：

NEUROCHEMICAL RESEARCH | 2010年 / 35卷 / 07期

关键词：

Calcium; Gelsolin; Mitogen-activated protein kinases; Protein kinase C; PROTEIN-KINASE-C; AMYLOID BETA-PROTEIN; ALZHEIMERS-DISEASE BRAIN; HYDROGEN-PEROXIDE; CASPASE-CLEAVAGE; APOPTOSIS; PATHOLOGY; COMPLEX; MICE; IDENTIFICATION;

D O I：

10.1007/s11064-010-0157-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gelsolin plays an important role in the regulation of amyloid beta-protein fibrillogenesis. We report here that calcium ionophore A23187 induces the expression of cytoplasmic gelsolin (c-gelsolin), and that protein kinase C (PKC) is involved in the up-regulation of c-gelsolin. In the presence of calcium, both SH-SY5Y and HEK-293 cells upon treatment with A23187 showed an increase in c-gelsolin expression in a concentration-dependent manner. Calcium-mediated up-regulation of c-gelsolin was inhibited by cycloheximide (a general inhibitor of protein synthesis). When cells were pretreated with staurosporine (an inhibitor of a variety of protein kinases including PKC), the up-regulation of c-gelsolin induced by A23187 was inhibited. Calphostin C, an inhibitor of PKC, blocked the up-regulation of c-gelsolin induced by A23187, while inhibitors of mitogen-activated protein kinases had no effect on c-gelsolin expression. In addition, phorbol-12-myristate-13-acetate, an activator of PKC, up-regulated c-gelsolin expression. These results suggest that calcium mediates up-regulation of c-gelsolin in a PKC-dependent manner.

引用

页码：1075 / 1082

页数：8

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