Quantum dots targeted to the assigned organelle in living cells

被引:167
作者
Hoshino, A
Fujioka, K
Oku, T
Nakamura, S
Suga, M
Yamaguchi, Y
Suzuki, K
Yasuhara, M
Yamamoto, K
机构
[1] Int Med Ctr Japan, Inst Res, Dept Med Ecol & Informat, Shinjuku Ku, Tokyo 1628655, Japan
[2] Tokyo Med & Dent Univ, Hosp Pharm, Dept Pharmacokinet & Pharmacodynam, Bunkyo Ku, Tokyo 1138519, Japan
[3] Natl Inst Infect Dis, Dept Bioact Mol, Tokyo 1628640, Japan
[4] Univ Tokyo, Sch Engn, Dept Chem Syst Engn, Bunkyo Ku, Tokyo 1138656, Japan
[5] Natl Inst Neurosci, Div Biochem & Cellular Biol, Kodaira, Tokyo 1878502, Japan
关键词
quantum dot; signal peptide; nanocrystal; nuclear localizing signal; mitochondria targeting signal; bioimaging;
D O I
10.1111/j.1348-0421.2004.tb03621.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Fluorescent nanocrystal quantum dots (QDs) have the potential to be applied to bioimaging since QDs emit higher and far longer fluorescence than conventional organic probes. Here we show that QDs conjugated with signal peptide obey the order to transport the assigned organelle in living cells. We designed the supermolecule of luminescent QDs conjugated with nuclear- and mitochondria-targeting ligands. When QDs with nuclear-localizing signal peptides were added to the culture media, we can visualize the movements of the QDs being delivered into the nuclear compartment of the cells with 15 min incubation. In addition, mitochondrial signal peptide can also transport QDs to the mitochondria in living cells. In conclusion, these techniques have the possibility that QDs can reveal the transduction of proteins and peptides into specific subcellular compartments as a powerful tool for studying intracellular analysis in vitro and even in vivo.
引用
收藏
页码:985 / 994
页数:10
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