Probing the canonicity of the Wnt/Wingless signaling pathway

被引:33
作者
Franz, Alexandra [1 ]
Shlyueva, Daria [2 ,3 ]
Brunner, Erich [1 ]
Stark, Alexander [2 ]
Basler, Konrad [1 ]
机构
[1] Univ Zurich, Inst Mol Life Sci, Zurich, Switzerland
[2] Vienna Bioctr VBC, Res Inst Mol Pathol IMP, Campus Vienna Bioctr 1, Vienna, Austria
[3] Univ Copenhagen, Fac Hlth & Med Sci, Biotech Res & Innovat Ctr, Copenhagen, Denmark
来源
PLOS GENETICS | 2017年 / 13卷 / 04期
基金
欧洲研究理事会; 奥地利科学基金会;
关键词
BETA-CATENIN; TRANSCRIPTIONAL ACTIVATION; ARMADILLO/BETA-CATENIN; DROSOPHILA; WINGLESS; GENE; TCF; REPRESSION; COMPLEX; BINDING;
D O I
10.1371/journal.pgen.1006700
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The hallmark of canonical Wnt signaling is the transcriptional induction of Wnt target genes by the beta-catenin/TCF complex. Several studies have proposed alternative interaction partners for beta-catenin or TCF, but the relevance of potential bifurcations in the distal Wnt pathway remains unclear. Here we study on a genome-wide scale the requirement for Armadillo (Arm, Drosophila beta-catenin) and Pangolin (Pan, Drosophila TCF) in the Wnt/Wingless(Wg)-induced transcriptional response of Drosophila Kc cells. Using somatic genetics, we demonstrate that both Arm and Pan are absolutely required for mediating activation and repression of target genes. Furthermore, by means of STARR-sequencing we identified Wnt/Wg-responsive enhancer elements and found that all responsive enhancers depend on Pan. Together, our results confirm the dogma of canonical Wnt/Wg signaling and argue against the existence of distal pathway branches in this system.
引用
收藏
页数:18
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