Human amnion-derived mesenchymal stem cells induced osteogenesis and angiogenesis in human adipose-derived stem cells via ERK1/2 MAPK signaling pathway

被引：13
作者：
Wang, Yuli [1 ,3 ]
Chen, Xichen [4 ]
Yin, Ying [5 ]
Li, Song [1 ,2 ]
机构：
[1] Nanjing Med Univ, Jiangsu Key Lab Oral Dis, Nanjing 210029, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp Stomatol, Dept Periodontol, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp Stomatol, Dept Oral & Maxillofacial Surg, Nanjing 210029, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Anal Ctr, Nanjing 211166, Jiangsu, Peoples R China
[5] Nanjing Univ, Med Sch, Nanjing Stomatol Hosp, Nanjing 210008, Jiangsu, Peoples R China
来源：
BMB REPORTS | 2018年 / 51卷 / 04期
关键词：
Angiogenesis; ERK1/2 MAPK signaling pathway; Human adipose-derived stem cells; Human amnion-derived mesenchymal stem cells; Osteogenesis; IN-VITRO; ENDOTHELIAL-CELLS; STROMAL CELLS; BONE REGENERATION; DIFFERENTIATION; ADIPONECTIN; TISSUE; OSTEOBLAST; MEMBRANE; INDUCTION;
D O I：
10.5483/BMBRep.2018.51.4.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs. Moreover, the positive effect of HAMSCs was significantly inhibited by U0126, a highly selective inhibitor of extracellular signaling-regulated kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) signaling pathway. These observations suggested that HAMSCs induced bone regeneration in HASCs via ERK1/2 MAPK signaling pathway.
引用
收藏
页码：194 / 199
页数：6
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