Microextraction by packed sorbent using a new restricted molecularly imprinted polymer for the determination of estrogens from human urine samples

被引：44

作者：

de Oliveira, Hanna Leijoto ^{[1
]}

Teixeira, Leila Suleimara ^{[1
]}

Fonseca Dinali, Laise Aparecida ^{[1
]}

Pires, Bruna Carneiro ^{[1
]}

Simoes, Nathalia Soares ^{[1
]}

Borges, Keyller Bastos ^{[1
]}

机构：

[1] Univ Fed Sao Joao del Rei UFSJ, Dept Ciencias Nat, Campus Dom Bosco,Praca Dom Helvecio 74, BR-36301160 Sao Joao Del Rei, MG, Brazil

来源：

MICROCHEMICAL JOURNAL | 2019年 / 150卷

关键词：

Microextraction by packed sorbent; Molecularly imprinted polymer; Restricted access material; Estriol; Estrone; Human urine; SOLID-PHASE EXTRACTION; BIOANALYTICAL METHOD VALIDATION; RATIONAL DESIGN; DRUG-DELIVERY; RECOGNITION; PERFORMANCE; GUIDELINES; ALBUMIN; WATER;

D O I：

10.1016/j.microc.2019.104162

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microextraction by packed sorbent (MEPS) was employed with a new restricted access molecularly imprinted polymer (MIP) for the determination of estrone (E1) and estriol (E3) from human urine samples. Several parameters affecting the extraction efficiency during the procedure of sample preparation were systematically optimized, including washing solvent, sample volume, amount of sorption material, elution solution, eluent volume, number of cycles of aspersion/dispersion and sample pH. Mean recoveries with their respective relative standard deviations (RSD%) for E1 and E3 were 79.5 +/- 7.1% and 69.5 +/- 1.5%, respectively. The method was linear in the concentration range of 100 to 1100 ng mL(-1) for each hormone, with correlation coefficient of 0.9954 and 0.9952 for E1 and E3, respectively. The developed method was successfully applied in urine sample from volunteer pregnant.

引用

页数：11

共 42 条

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