Improved in vitro clonal propagation of Rauwolfia serpentina L. Benth - An endangered medicinal plant

被引：9

作者：

Senapati, S. K. ^{[1
]}

Lahere, N. ^{[1
]}

Tiwary, B. N. ^{[1
]}

机构：

[1] Guru Ghasidas Vishwavidyalaya, Dept Biotechnol, Bilaspur 495009, Chhattisgarh, India

来源：

PLANT BIOSYSTEMS | 2014年 / 148卷 / 05期

关键词：

micropropagation; cytokinins; auxins;

D O I：

10.1080/11263504.2013.845264

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this protocol, 96% of bud proliferation was observed after 14 days of inoculation. Highest rate of shoot multiplication (13.3 shoots per explant) with an average shoot length of 8.2cm was observed in Murashige and Skoog (MS) medium+2mg/l 6-benzylaminopurine+0.25mg/l1-naphthaleneacetic acid+3% sucrose. Half MS (1/2 MS)+0.5mg/l indol-3-butyric acid proved best with 73.3% rooting. The rooted shoots showed 90% survival. Genetic uniformity of the micropropagated plants with their donor plants was confirmed through random amplified polymorphic DNA molecular technique.

引用

页码：885 / 888

页数：4

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