EXPRESSION OF CD152 (CTLA-4) IN CHILDREN WITH AUTOIMMUNE THYROIDITIS AND+49 A/G POLYMORPHISM OF EXON 1 OF THE CTLA-4 GENE

被引：0

作者：

Kucharska, A. M. ^{[1
]}

Gorska, E. ^{[2
]}

Wasik, M. ^{[2
]}

Pyrzak, B. ^{[1
]}

Demkow, U. ^{[2
]}

机构：

[1] Warsaw Med Univ, Dept Paediat & Endocrinol, Warsaw, Poland

[2] Warsaw Med Univ, Dept Lab Diagnost & Clin Immunol Dev Age, Warsaw, Poland

来源：

JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 2009年 / 60卷

关键词：

CTLA-4; Hashimoto's thyroiditis; polymorphism; T cell; homozygotes; T-LYMPHOCYTE ANTIGEN-4; CD28; SUPERFAMILY; DISEASE; SEQUENCE; SURFACE;

D O I：

暂无

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

CTLA-4 gene is one of the strongest locus of genetic susceptibility to autoimmune thyroid diseases. The aim of the present study was to investigate surface expression of CTLA-4 on peripheral T cells in homozygotes AA and GG at position +49 of CTLA-4 gene in children with Hashimoto's thyroiditis and in healthy controls. Blood samples were obtained from 100 children: 45 with Hashimoto's thyroiditis and 55 controls. CTLA-4 exon 1 polymorphism was defined by SSCP and RFLP with BbvI enzyme. T cells were analyzed with three color flow cytometry by Coulter EPICS XL. We found that CTLA-4 expression was significantly lower in the thyroiditis patients than in controls, but CTLA-4 expression in homozygotes GG and AA was comparable. We therefore conclude that decreased expression of CTLA-4 on T cells in children with Hashimoto's thyroiditis is not dependent on polymorphic changes at position +49 of CTLA-4 gene.

引用

页码：77 / 80

页数：4

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