Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma

被引：22
作者：
Zhang, Zuojun [1 ,2 ]
Zhao, Ming [1 ]
Wang, Guojie [1 ]
机构：
[1] Luoyang Orthoped Hosp Henan Prov, Upper Limb Injury Treatment Ctr, Luoyang, Peoples R China
[2] Luoyang Orthoped Hosp Henan Prov, Orthoped Hosp Henan Prov, 82 Qiming South Rd, Luoyang 122400, Henan, Peoples R China
来源：
CELL AND BIOSCIENCE | 2019年 / 9卷 / 01期
关键词：
Hsa_circ_0051079; miR-26a-5p; TGF-beta; 1; Osteosarcoma; GROWTH-FACTOR BETA-1; CIRCULAR RNA; INVASION; CANCER; PROLIFERATION; METASTASIS; EXPRESSION; MIGRATION; MIRNA;
D O I：
10.1186/s13578-019-0355-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background Osteosarcoma is a most common bone malignant tumor which threatens children and adolescents. Circular RNAs (circRNAs) fundamentally play essential roles in the progress and development of human cancers by sponging with microRNAs (miRNAs). However, the role of circRNAs in osteosarcoma is not clear. The aim of the study was to investigate the roles and molecular mechanism of circRNAs in osteosarcoma. Results The data from qRT-PCR showed that circ_0051079 expression was higher in osteosarcoma cells and tissues compared to their normal controls. Meanwhile, bioinformatic analysis indicated that circ_0051079 was a sponge of miR-26a-5p, which was verified by luciferase activity assay. Subsequently, TGF-beta 1 was verified as a putative target mRNA of miR-26a-5p by luciferase assay. Cellular function assays were conducted and the findings revealed that circ_0051079/miR-26a-5p/TGF-beta 1 regulated osteosarcoma proliferation and metastasis. Conclusion The study demonstrated that circ_0051079 could act as an oncogene via regulating miR-26a-5p/TGF-beta 1 and a potential biomarker for osteosarcoma diagnose.
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页数：11
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