Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach

被引：22

作者：

Katayama, Tetsuro

Shimamoto, Shohei

Oda, Hideyuki

Nakahara, Keiko

Kangawa, Kenji

Murakami, Noboru

机构：

[1] Miyazaki Univ, Frontier Sci Res Ctr, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Fac Agr, Dept Vet Physiol, Miyazaki 8892192, Japan

[3] Natl Cardiovasc Ctr, Res Inst, Osaka 5658565, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 04期

关键词：

ghrelin; desacyl-ghrelin; glucagon receptor; stomach; quantitative RT-PCR; double staining; rat;

D O I：

10.1016/j.bbrc.2007.04.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was performed to evaluate the role of glucagon in the regulation of ghrelin secretion from the rat stomach. mRNA for ghrelin and glucagon receptor was expressed predominantly in the lower body and pylorus of stomach, but little or not in the upper body and cardia. Ghrelin- and glucagon receptor-immunoreactive cells were detected in lamina propria mucosae of stomach and some cells expressed both. Intravenous administration of glucagon caused transient increases in both acyl- and desacyl-ghrelin levels in the gastric vein within 10 min, which was followed by gradual increases in desacyl-ghrelin levels until 60 min. Steady state levels of ghrelin mRNA in the stomach were increased by 1.9-fold 20 min after glucagon administration, but not at 5 or 120 min. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：865 / 870

页数：6

共 22 条

[1] REGULATION OF GLUCAGON AND GLUCAGON-LIKE PEPTIDE-1 RECEPTOR MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN CULTURED RAT PANCREATIC-ISLETS BY GLUCOSE, CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE, AND GLUCOCORTICOIDS [J].