Identification of potential markers and sensitive tissues for low or high salinity stress in an intertidal mud crab (Macrophthalmus japonicus)

被引：38
作者：
Nikapitiya, Chamilani [1 ,2 ]
Kim, Won-Seok [2 ]
Park, Kiyun [2 ]
Kwak, Ihn-Sil [2 ]
机构：
[1] Chonnam Natl Univ, Dept Aqualife Med, Chungnam 550749, South Korea
[2] Chonnam Natl Univ, Fac Marine Technol, Chungnam 550749, South Korea
来源：
FISH & SHELLFISH IMMUNOLOGY | 2014年 / 41卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Arginine kinase; Ecdysteroid receptor; LGBP; Macrophthalmus japonicus; Salinity change; SHRIMP LITOPENAEUS-VANNAMEI; DIFFERENTIALLY EXPRESSED GENES; WHITE SHRIMP; ARGININE KINASE; MOLECULAR-CLONING; PENAEUS-VANNAMEI; IMMUNE-RESPONSE; MOLT CYCLE; BLUE-CRAB; HEPATOPANCREAS;
D O I：
10.1016/j.fsi.2014.09.018
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Macrophthalmus japonicus is an intertidal mud crab is an ecologically important species in Korea, can tolerate a wide range of natural and anthropogenic stressors. Environmental changes especially salinity cause physiological stress to the marine habitats. Differential gene transcription of M. japonicus tissues provided information about tissue specific responses against salinity. Five potential genes were identified and their transcription levels were determined quantitatively comparison to seawater (SW: 31 +/- 1 psu) in M. japonicus gills and hepatopancreas after exposed them to different salinities. Ecdysteroid receptor (Mj-EcR), trypsin (Mj-Tryp), arginine kinase (Mj-AK), lipopolysaccharide and beta-1,3-glucan binding protein (Mj-LGBP) and peroxinectin (Mj-Prx) in hepatopancreas up-regulated against different salinities. In contrast, the gills, Mj-EcR, Mj-Tryp and Mj-AK showed late up-regulated responses to 40 psu compared to SW. All genes except Mj-LGBP showed up regulation in the gills as time dependent manner. These genes can be considered as potential markers to assess responses in salinity changes. This study suggests hepatopancreas is a suitable tissue for transcriptional, biochemical and physiological responses analysis on M. japonicus in low and high salinity stress. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：407 / 416
页数：10
相关论文
共 67 条
[1]   Metabolic responses and arginine kinase expression under hypoxic stress of the kuruma prawn Marsupenaeus japonicus [J].
Abe, Hiroki ;
Hirai, Shun ;
Okada, Shigeru .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2007, 146 (01) :40-46
[2]   Identification and Characterization of Differentially Expressed Transcripts in the Gills of Freshwater Prawn (Macrobrachium rosenbergii) under Salt Stress [J].
Barman, Hirak Kumar ;
Patra, Swagat Kumar ;
Das, Varsha ;
Mohapatra, Shibani Dutta ;
Jayasankar, Pallipuram ;
Mohapatra, Chinmayee ;
Mohanta, Ramya ;
Panda, Rudra Prasanna ;
Rath, Surya Narayan .
SCIENTIFIC WORLD JOURNAL, 2012,
[3]   The conceptual approach to lobster shell disease revisited [J].
Castro, Kathleen M. ;
Factor, Jan Robert ;
Angell, Thomas ;
Landers, Donald F., Jr. .
JOURNAL OF CRUSTACEAN BIOLOGY, 2006, 26 (04) :646-660
[4]   Mass mortality in Pacific oysters is associated with a specific gene expression signature [J].
Chaney, Maxine L. ;
Gracey, Andrew Y. .
MOLECULAR ECOLOGY, 2011, 20 (14) :2942-2954
[5]   The transcriptomic responses of the eastern oyster, Crassostrea virginica, to environmental conditions [J].
Chapman, Robert W. ;
Mancia, Annalaura ;
Beal, Marion ;
Veloso, Artur ;
Rathburn, Charles ;
Blair, Anne ;
Holland, A. F. ;
Warr, G. W. ;
Didinato, Guy ;
Sokolova, Inna M. ;
Wirth, Edward F. ;
Duffy, Edward ;
Sanger, Denise .
MOLECULAR ECOLOGY, 2011, 20 (07) :1431-1449
[6]   Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain [J].
Chung, Kuei-Fang ;
Lin, Hui-Chen .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2006, 144 (01) :48-57
[7]   Stress-induced gene expression profiling in the black tiger shrimp Penaeus monodon [J].
de la Vega, Enrique ;
Hall, Michael R. ;
Wilson, Kate J. ;
Reverter, Antonio ;
Woods, Rick G. ;
Degnan, Bernard M. .
PHYSIOLOGICAL GENOMICS, 2007, 31 (01) :126-138
[8]  
EISEN AZ, 1969, BIOCHIM BIOPHYS ACTA, V191, P517
[9]   Evolution and physiological roles of phosphagen systems [J].
Ellington, WR .
ANNUAL REVIEW OF PHYSIOLOGY, 2001, 63 :289-325
[10]   Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology [J].
Feder, ME ;
Hofmann, GE .
ANNUAL REVIEW OF PHYSIOLOGY, 1999, 61 :243-282
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