Identification of five Orius species in Japan by multiplex polymerase chain reaction

被引：36

作者：

Hinomoto, N ^{[1
]}

Muraji, M

Noda, T

Shimizu, T

Kawasaki, K

机构：

[1] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058634, Japan

[2] Ryukyu Sankei Co Ltd, Okinawa 9030814, Japan

来源：

BIOLOGICAL CONTROL | 2004年 / 31卷 / 03期

关键词：

Orius sauteri; Orius minutus; Orius strigicollis; Orius nagaii; Orius tantillus; multiplex polymerase chain reaction; species identification; internal transcribed spacer 1; ribosomal DNA;

D O I：

10.1016/j.biocontrol.2004.07.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed multiplex polymerase chain reaction methods to identify five Orius (Heteroptera: Anthocoridae) species that occur commonly in Japan: Orius sauteri, Orius minutus, Orius strigicollis, Orius nagaii, and Orius tantillus. The method amplified internal transcribed spacer 1 of the nuclear ribosomal DNA by using five primers simultaneously and produced species-specific banding patterns upon agarose gel electrophoresis. Reliability of the method was tested for 350 individuals of 23 strains, and consistent results were obtained. Dichotomous keys are also provided for easy and quick species identification. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：276 / 279

页数：4

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