Hybrid single-source online Fourier transform coherent anti-Stokes Raman scattering/optical coherence tomography

被引：12

作者：

Kamali, Tschackad ^{[1
,2
]}

Povazay, Boris ^{[1
,2
]}

Kumar, Sunil ^{[3
]}

Silberberg, Yaron ^{[3
]}

Hermann, Boris ^{[1
,2
]}

Werkmeister, Rene ^{[1
,2
]}

Drexler, Wolfgang ^{[1
,2
]}

Unterhuber, Angelika ^{[1
,2
]}

机构：

[1] Med Univ Vienna, Ctr Med Phys & Biomed Engn, A-1090 Vienna, Austria

[2] Med Univ Vienna, Med Imaging Cluster, A-1090 Vienna, Austria

[3] Weizmann Inst Sci, Dept Phys Complex Syst, IL-761000 Rehovot, Israel

来源：

OPTICS LETTERS | 2014年 / 39卷 / 19期

关键词：

HIGH-SENSITIVITY; CARS MICROSCOPY; SPECTROSCOPY; EXTRACTION; RESOLUTION; SPECTRA; LASER;

D O I：

10.1364/OL.39.005709

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a multimodal optical coherence tomography (OCT) and online Fourier transform coherent anti-Stokes Raman scattering (FTCARS) platform using a single sub-12 femtosecond (fs) Ti: sapphire laser enabling simultaneous extraction of structural and chemical ("morphomolecular") information of biological samples. Spectral domain OCT prescreens the specimen providing a fast ultrahigh (4 x 12 mu m axial and transverse) resolution wide field morphologic overview. Additional complementary intrinsic molecular information is obtained by zooming into regions of interest for fast label-free chemical mapping with online FTCARS spectroscopy. Background-free CARS is based on a Michelson interferometer in combination with a highly linear piezo stage, which allows for quick point-to-point extraction of CARS spectra in the fingerprint region in less than 125 ms with a resolution better than 4 cm(-1) without the need for averaging. OCT morphology and CARS spectral maps indicating phosphate and carbonate bond vibrations from human bone samples are extracted to demonstrate the performance of this hybrid imaging platform. (C) 2014 Optical Society of America

引用

页码：5709 / 5712

页数：4

共 29 条

[1] Multi-modal approach using Raman spectroscopy and optical coherence tomography for the discrimination of colonic adenocarcinoma from normal colon [J].