Optimization of nutritional requirements and feeding strategies for clavulanic acid production by Streptomyces clavuligerus

被引：54

作者：

Saudagar, Parag S. ^{[1
]}

Singhal, Rekha S. ^{[1
]}

机构：

[1] Univ Bombay, Inst Chem Technol, Food Engn & Technol Dept, Bombay 400019, Maharashtra, India

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 10期

关键词：

clavulanic acid; fed-batch fermentation; Streptomyces clavuligerus; threonine; arginine;

D O I：

10.1016/j.biortech.2006.08.003

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The present work reports the nutritional requirements and environmental conditions for submerged culture of Streptomyces clavuligerus for clavulanic acid production using orthogonal matrix method (Taguchi L-16 design) and also fed-batch fermentation for clavulanic acid production by feeding glycerol, arginine and threonoine to the fermentation medium intermittently. Clavulanic acid production was increased by 18% with the span of feeding glycerol and reached a maximum at 1.30 mg/ml with 120 h glycerol feeding as compared to 1.10 mg/ml in the control. The production also increased with the span of feeding amino acids and reached a maximum of 1.31 and 1.86 mg/ml with feeding arginine and threonine, respectively in 120 h. There was an overall increase of 18% and 9% in clavulanic acid production with arginine and threonine feeding as compared to the respective controls (1.10 and 1.70 mg/ml, respectively). (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2010 / 2017

页数：8

共 31 条

[1] CARBON CATABOLITE REGULATION OF CEPHALOSPORIN PRODUCTION IN STREPTOMYCES CLAVULIGERUS [J].

AHARONOWITZ, Y ;

DEMAIN, AL .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1978, 14 (02) :159-164

[2] Chemistry and biosynthesis of clavulanic acid and other clavams [J].

Baggaley, KH ;

Brown, AG ;

Schofield, CJ .

NATURAL PRODUCT REPORTS, 1997, 14 (04) :309-333

[3]

BHATTACHARJEE S, 2002, INDIAN J MICROBIOL, V42, P67

[4] Enhancement of clavulanic acid production in Streptomyces clavuligerus with ornithine feeding [J].

Chen, KC ;

Lin, YH ;

Wu, JY ;

Hwang, SCJ .

ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (01) :152-156

[5] Optimization of glycerol feeding for clavulanic acid production by Streptomyces clavuligerus with glycerol feeding [J].

Chen, KC ;

Lin, YH ;

Tsai, CM ;

Hsieh, CH ;

Houng, JY .

BIOTECHNOLOGY LETTERS, 2002, 24 (06) :455-458

[6] MICRODETERMINATION OF PHOSPHORUS [J].

CHEN, PS ;

TORIBARA, TY ;

WARNER, H .

ANALYTICAL CHEMISTRY, 1956, 28 (11) :1756-1758

[7] Fed-batch enhancement of jenseniin G, a bacteriocin produced by Propionibacterium thoenii (jensenii) P126 [J].

Ekinci, FY ;

Barefoot, SF .

FOOD MICROBIOLOGY, 2006, 23 (04) :325-330

[8] STUDIES ON BIOSYNTHESIS OF CLAVULANIC ACID .1. INCORPORATION OF C-13-LABELLED PRECURSORS [J].

ELSON, SW ;

OLIVER, RS .

JOURNAL OF ANTIBIOTICS, 1978, 31 (06) :586-592

[9] ASSAY OF AMOXICILLIN AND CLAVULANIC ACID, THE COMPONENTS OF AUGMENTIN, IN BIOLOGICAL-FLUIDS WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

FOULSTONE, M ;

READING, C .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (05) :753-762

[10] Improvement of clavulanic acid production by Streptomyces clavuligerus in medium containing soybean derivatives [J].

Gouveia, ER ;

Baptista-Neto, A ;

Azevedo, AG ;

Badino, AC ;

Hokka, CO .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1999, 15 (05) :623-627

← 1 2 3 4 →