Hydrogenotrophic Sulfate Reduction in a Gas-Lift Bioreactor Operated at 9°C

被引:8
作者
Nevatalo, Laura M. [1 ]
Bijmans, Martijn F. M. [2 ]
Lens, Piet N. L. [2 ]
Kaksonen, Anna H. [1 ]
Puhakka, Jaakko A. [1 ]
机构
[1] Tampere Univ Technol, Dept Chem & Bioengn, FIN-33101 Tampere, Finland
[2] Univ Wageningen & Res Ctr, Subdept Environm Technol, NL-6700 EV Wageningen, Netherlands
基金
芬兰科学院;
关键词
Sulfate reduction; low temperature; gas-lift bioreactor; hydrogenotroph; homoacetogenesis; REDUCING BACTERIA; RETENTION TIME; CARBON-DIOXIDE; GROWTH-RATE; SP-NOV; TEMPERATURE; METHANOGENESIS; OXIDATION; SULFIDE; METAL;
D O I
10.4014/jmb.0906.06016
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The viability of low-temperature sulfate reduction with hydrogen as electron donor was studied with a bench-scale gas-lift bioreactor (GLB) operated at 9 degrees C. Prior to the GLB experiment, the temperature range of sulfate reduction of the inoculum was assayed. The results of the temperature gradient assay indicated that the inoculum was a psychrotolerant mesophilic enrichment culture that had an optimal temperature for sulfate reduction of 31 degrees C, and minimum and maximum temperatures of 7 degrees C and 41 degrees C, respectively. In the GLB experiment at 9 degrees C, a sulfate reduction rate of 500-600 mg l(-1) d(-1), corresponding to a specific activity of 173 mg SO42- g VSS-1 d(-1), was obtained. The electron flow from the consumed H-2-gas to sulfate reduction varied between 27% and 52%, whereas the electron flow to acetate production decreased steadily from 15% to 5%. No methane was produced. Acetate was produced from CO2 and H-2 by homoacetogenic bacteria. Acetate supported the growth of some heterotrophic sulfate-reducing bacteria. The sulfate reduction rate in the GLB was limited by the slow biomass growth rate at 9 degrees C and low biomass retention in the reactor. Nevertheless, this study demonstrated the potential sulfate reduction rate of psychrotolerant sulfate-reducing mesophiles at suboptimal temperature.
引用
收藏
页码:615 / 621
页数:7
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