A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus

被引：189

作者：

Chen, X ^{[1
]}

Stone, M ^{[1
]}

Schlagnhaufer, C ^{[1
]}

Romaine, CP ^{[1
]}

机构：

[1] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 10期

关键词：

D O I：

10.1128/AEM.66.10.4510-4513.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.

引用

页码：4510 / 4513

页数：4

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