Interleukin-6-induced tyrosine phosphorylation of phospholipase C-γ1 in PC12 cells

Phospholipase C (PLC)-gamma 1 plays a pivotal role in the signal transduction pathway mediated by growth factors. In this study, we found that neurite outgrowth of pheochromocytoma (PC12) cells was significantly induced by interleukin-6 (IL-6). Stimulation of PC12 cells with IL-6 led to tyrosine phosphorylation of PLC-gamma 1 in a dose- and time-dependent manner. IL-6 stimulation also increased the hydrolysis of phosphatidylinositol 4,5-bisphosphate. Accumulation of total inositol phosphate as well as tyrosine phosphorylation of PLC-gamma 1 was inhibited by the pretreatment of protein kinase inhibitors such as genistein and staurosporine, These results suggest-that PLC-gamma 1 may be involved in the signal transduction pathway of IL-6-induced PC12 cell differentiation.