Genetic transformation of Coniochaeta sp. 2T2.1, key fungal member of a lignocellulose-degrading microbial consortium

被引：1
作者：
Nichols, Nancy N. [1 ]
Hector, Ronald E. [1 ]
Frazer, Sarah E. [1 ]
机构：
[1] ARS, Natl Ctr Agr Utilizat Res, USDA, Bioenergy Res Unit, Peoria, IL USA
来源：
BIOLOGY METHODS & PROTOCOLS | 2019年 / 4卷 / 01期
关键词：
transformation; protoplast; GFP; fungi; biomass;
D O I：
10.1093/biomethods/bpz001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Coniochaeta sp. strain 2T2.1 is a key member of a microbial consortium that degrades lignocellulosic biomass. Due to its ecological niche and ability to also grow in pure culture on wheat straw, protocols for transformation and antibiotic selection of the strain were established. Hygromycin was found to be a reliable selectable transformation marker, and the mammalian codon-optimized green fluorescent protein was expressed and used to visualize fluorescence in transformed cells of strain 2T2.1.
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页数：5
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