Reversal of multidrug resistance of vincristine-resistant gastric adenocarcinoma cells through up-regulation of DARPP-32

被引：14

作者：

Hong, Liu ^{[1
]}

Wang, Jin ^{[1
]}

Han, Ying ^{[1
]}

Zhao, Yunping ^{[1
]}

Gao, Juan ^{[1
]}

Wang, Jun ^{[1
]}

Han, Yu ^{[1
]}

Zhang, Xiaoyin ^{[1
]}

Yan, Li ^{[1
]}

Zhou, Xinmin ^{[1
]}

Qiao, Taidong ^{[1
]}

Chen, Zhen ^{[1
]}

Fan, Daiming ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, State Key Lab Canc Biol, Xian 710032, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2007年 / 31卷 / 09期

关键词：

DARPP-32; multidrug resistance; gastric neoplasm; ZNRD1; P-gp;

D O I：

10.1016/j.cellbi.2007.03.020

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Here we investigated the roles of DARPP-32 in multidrug resistance (MDR) of gastric cancer cells and the possible underlying mechanisms. We constructed the eukaryotic expression vector of DARPP-32 and transfected it into human vincristine-resistant gastric adenocarcinoma cell line SGC7901/VCR. Up-regulation of DARPP-32 could significantly enhance the sensitivity of SGC7901/VCR cells towards vincristine, adriamycin, 5-fluorouracil and cisplatin, and could decrease the capacity of cells to efflux adriamycin. What's more, the results of subrenal capsule assay confirmed that DARPP-32 might play a certain role in MDR of gastric cancer. DARPP-32 could significantly down-regulate the expression of P-gp and zinc ribbon domain-containing 1 (ZNRD1), but not alter the expression of multidrug resistance-associated protein (MRP) or the glutathione S-transferase (GST). DARPP-32 could also significantly decrease the anti-apoptotic activity of SGC7901/VCR cells. Further study of the biological functions of DARPP-32 might be helpful for understanding the mechanisms of MDR in gastric cancer. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：1010 / 1015

页数：6

共 17 条

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