Discovery of a Gene Conferring Multiple-Aminoglycoside Resistance in Escherichia coli

被引：91

作者：

Davis, Margaret A. ^{[1
]}

Baker, Katherine N. K. ^{[1
]}

Orfe, Lisa H. ^{[1
]}

Shah, Devendra H. ^{[1
]}

Besser, Thomas E. ^{[1
]}

Call, Douglas R. ^{[1
]}

机构：

[1] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 06期

基金：

美国国家卫生研究院;

关键词：

16S RIBOSOMAL-RNA; ANTIMICROBIAL RESISTANCE; STREPTOMYCES-GRISEUS; DNA MICROARRAY; SALMONELLA; METHYLASES; SEQUENCE; CLUSTER; CLONING; ARMA;

D O I：

10.1128/AAC.01743-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine-origin Escherichia coli isolates were tested for resistance phenotypes using a disk diffusion assay and for resistance genotypes using a DNA microarray. An isolate with gentamicin and amikacin resistance but with no corresponding genes detected yielded a 1,056-bp DNA sequence with the closest homologues for its inferred protein sequence among a family of 16S rRNA methyltransferase enzymes. These enzymes confer high-level aminoglycoside resistance and have only recently been described in Gram-negative bacteria.

引用

页码：2666 / 2669

页数：4

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