Confocal laser scanning microscopy with spatiotemporal structured illumination

被引:23
作者
Gao, Peng [1 ,2 ]
Nienhaus, G. Ulrich [1 ,2 ,3 ]
机构
[1] Karlsruhe Inst Technol, Inst Appl Phys, D-76128 Karlsruhe, Germany
[2] Karlsruhe Inst Technol, Inst Nanotechnol, D-76344 Eggenstein Leopoldshafen, Germany
[3] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
关键词
FOCAL MODULATION MICROSCOPY; RESOLUTION; SUPERRESOLUTION; OPTICS; LIGHT; FIELD;
D O I
10.1364/OL.41.001193
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Confocal laser scanning microscopy (CLSM), which is widely utilized in the biological and biomedical sciences, is limited in spatial resolution due to diffraction to about half the light wavelength. Here we have combined structured illumination with CLSM to enhance its spatial resolution. To this end, we have used a spatial light modulator (SLM) to generate fringe patterns of different orientations and phase shifts in the excitation spot without any mechanical movement. We have achieved 1.8 and 1.7 times enhanced lateral and axial resolutions, respectively, by synthesizing the object spectrum along different illumination directions. This technique is thus a promising tool for high-resolution morphological or fluorescence imaging, especially in deep tissue. (C) 2016 Optical Society of America
引用
收藏
页码:1193 / 1196
页数:4
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