Peripheral Blood from Rheumatoid Arthritis Patients Shows Decreased Treg CD25 Expression and Reduced Frequency of Effector Treg Subpopulation

被引：21

作者：

Go, Eunbyeol ^{[1
,2
]}

Yoo, Su-Jin ^{[3
]}

Choi, Suyoung ^{[1
,2
,4
]}

Sun, Pureum ^{[1
,2
]}

Jung, Min Kyung ^{[5
]}

Kwon, Somin ^{[6
]}

Heo, Bu Yeon ^{[1
,2
,4
]}

Kim, Yeeun ^{[1
,2
]}

Kang, Ju-Gyeong ^{[7
]}

Kim, Jinhyun ^{[3
]}

Shin, Eui-Cheol ^{[5
,8
]}

Kang, Seong Wook ^{[3
]}

Kwon, Jaeyul ^{[1
,2
,4
,9
,10
]}

机构：

[1] Chungnam Natl Univ, Dept Med Sci, Coll Med, Daejeon 35015, South Korea

[2] Chungnam Natl Univ, Dept Infect Biol, Coll Med, Daejeon 35015, South Korea

[3] Chungnam Natl Univ, Dept Internal Med, Coll Med, Daejeon 35015, South Korea

[4] Chungnam Natl Univ, Brain Korea 21 FOUR Project Med Sci, Daejeon 35015, South Korea

[5] Korea Adv Inst Sci & Technol KAIST, Grad Sch Med Sci & Engn, Lab Immunol & Infect Dis, Daejeon 34141, South Korea

[6] NIA, Lab Neurogenet, NIH, Bethesda, MD 20892 USA

[7] Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea

[8] KAIST Inst, Ctr Epidem Preparedness, Daejeon 34141, South Korea

[9] Chungnam Natl Univ, Dept Med Educ, Coll Med, Daejeon 35015, South Korea

[10] Chungnam Natl Univ, Translat Immunol Inst, Daejeon 35015, South Korea

来源：

CELLS | 2021年 / 10卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

rheumatoid arthritis; regulatory T cells; CD25; Foxp3; effector regulatory T cells; CD127; naï ve regulatory T cells; CD45RA; FUNCTIONAL DEFECTS; CELL DYSFUNCTION; SYNOVIAL-FLUID; TREG CELLS; INTERLEUKIN-2; AUTOIMMUNE; FOXP3; DISEASE; TOLERANCE; PHENOTYPE;

D O I：

10.3390/cells10040801

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rheumatoid arthritis (RA) is a common autoimmune disease characterized by immune cell infiltration of the synovium, leading to the loss of cartilage, bone, and joint function. Although regulatory T (T-reg) cells are thought to modulate the initiation and progression of RA, a consensus has yet to be reached regarding the function and composition of T-reg cells in RA patients. To address these discrepancies, we analyzed not only the total T-reg frequency but also that of T-reg subpopulations in the peripheral blood of RA patients and healthy controls by flow cytometry. We found that the total T-reg population was not significantly different between RA and control subjects. However, the effector T-reg cell subgroup, defined as CD45RA(-)CD25(hi), showed markedly decreased frequency in RA patients. In addition, the total T-reg population from RA patients showed a significant decline in the expression of CD25. Both the naive and effector T-reg subgroups also showed marked reduction of CD25 expression in RA patients compared to controls. These data suggest that the decreased frequency of effector T-reg cells and overall reduction of CD25 expression in T-reg cells in the peripheral blood may be evidence of altered T-reg homeostasis associated with RA pathogenesis.

引用

页数：13