Interaction of influenza virus NS1 protein with growth arrest-specific protein 8

被引:7
作者
Zhao, Lixia [1 ,2 ]
Xu, Long [1 ]
Zhou, Xiaowei [1 ]
Zhu, Qingyu [2 ]
Yang, Zhixin [1 ]
Zhang, Chuanfu [1 ]
Zhu, Xudong [1 ]
Yu, Mengbin [1 ]
Zhang, Yingying [1 ]
Zhao, Xinghui [2 ]
Huang, Peitang [1 ]
机构
[1] Beijing Inst Biotechnol, Lab Prot Engn, Beijing 100071, Peoples R China
[2] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
关键词
CELLS; NUCLEAR; GAS11; RAB3B; GENE; ASSOCIATION; DREBRIN; BINDS;
D O I
10.1186/1743-422X-6-218
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
NS1 protein is the only non-structural protein encoded by the influenza A virus, and it contributes significantly to disease pathogenesis by modulating many virus and host cell processes. A two-hybrid screen for proteins that interact with NS1 from influenza A yielded growth arrest-specific protein 8. Gas8 associated with NS1 in vitro and in vivo. Deletion analysis revealed that the N-terminal 260 amino acids of Gas8 were able to interact with NS1, and neither the RNA-binding domain nor the effector domain of NS1 was sufficient for the NS1 interaction. We also found that actin, myosin, and drebrin interact with Gas8. NS1 and beta-actin proteins could be co-immunoprecipitated from extracts of transfected cells. Furthermore, actin and Gas8 co-localized at the plasma membrane. These results are discussed in relation to the possible functions of Gas8 protein and their relevance in influenza virus release.
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页数:9
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