Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review

被引：76

作者：

Chen, Xin-Fei ^{[1
,2
,3
]}

Hou, Xin ^{[1
,3
]}

Xiao, Meng ^{[1
,3
]}

Zhang, Li ^{[1
,3
]}

Cheng, Jing-Wei ^{[4
]}

Zhou, Meng-Lan ^{[1
,3
]}

Huang, Jing-Jing ^{[1
,2
,3
]}

Zhang, Jing-Jia ^{[1
,3
]}

Xu, Ying-Chun ^{[1
,3
]}

Hsueh, Po-Ren ^{[5
,6
,7
,8
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, State Key Lab Complex Severe & Rare Dis, Dept Lab Med, Beijing 100730, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China

[3] Beijing Key Lab Mech Res & Precis Diag Invas Fung, Beijing 100730, Peoples R China

[4] Capital Med Univ, Beijing Friendship Hosp, Ctr Clin Lab, Beijing 100053, Peoples R China

[5] China Med Univ, China Med Univ Hosp, Sch Med, Dept Lab Med, Taichung 40447, Taiwan

[6] China Med Univ, China Med Univ Hosp, Sch Med, Dept Internal Med, Taichung 40447, Taiwan

[7] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Coll Med, Dept Lab Med, Taipei 100, Taiwan

[8] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Coll Med, Dept Internal Med, Taipei 100, Taiwan

来源：

MICROORGANISMS | 2021年 / 9卷 / 07期

基金：

中国国家自然科学基金;

关键词：

matrix-assisted laser desorption; ionization-time-of-flight mass spectrometry (MALDI-TOF MS); pathogenic microorganism identification; DESORPTION-IONIZATION-TIME; URINARY-TRACT PATHOGENS; GRAM-NEGATIVE BACTERIA; POSITIVE BLOOD CULTURE; ROUTINE IDENTIFICATION; SPECIES IDENTIFICATION; RAPID IDENTIFICATION; SUBSPECIES IDENTIFICATION; MULTICENTER EVALUATION; BACTEROIDES-FRAGILIS;

D O I：

10.3390/microorganisms9071536

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.

引用

页数：16

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