Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review

被引：76
作者：
Chen, Xin-Fei [1 ,2 ,3 ]
Hou, Xin [1 ,3 ]
Xiao, Meng [1 ,3 ]
Zhang, Li [1 ,3 ]
Cheng, Jing-Wei [4 ]
Zhou, Meng-Lan [1 ,3 ]
Huang, Jing-Jing [1 ,2 ,3 ]
Zhang, Jing-Jia [1 ,3 ]
Xu, Ying-Chun [1 ,3 ]
Hsueh, Po-Ren [5 ,6 ,7 ,8 ]
机构：
[1] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, State Key Lab Complex Severe & Rare Dis, Dept Lab Med, Beijing 100730, Peoples R China
[2] Chinese Acad Med Sci & Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China
[3] Beijing Key Lab Mech Res & Precis Diag Invas Fung, Beijing 100730, Peoples R China
[4] Capital Med Univ, Beijing Friendship Hosp, Ctr Clin Lab, Beijing 100053, Peoples R China
[5] China Med Univ, China Med Univ Hosp, Sch Med, Dept Lab Med, Taichung 40447, Taiwan
[6] China Med Univ, China Med Univ Hosp, Sch Med, Dept Internal Med, Taichung 40447, Taiwan
[7] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Coll Med, Dept Lab Med, Taipei 100, Taiwan
[8] Natl Taiwan Univ, Natl Taiwan Univ Hosp, Coll Med, Dept Internal Med, Taipei 100, Taiwan
来源：
MICROORGANISMS | 2021年 / 9卷 / 07期
基金：
中国国家自然科学基金;
关键词：
matrix-assisted laser desorption; ionization-time-of-flight mass spectrometry (MALDI-TOF MS); pathogenic microorganism identification; DESORPTION-IONIZATION-TIME; URINARY-TRACT PATHOGENS; GRAM-NEGATIVE BACTERIA; POSITIVE BLOOD CULTURE; ROUTINE IDENTIFICATION; SPECIES IDENTIFICATION; RAPID IDENTIFICATION; SUBSPECIES IDENTIFICATION; MULTICENTER EVALUATION; BACTEROIDES-FRAGILIS;
D O I：
10.3390/microorganisms9071536
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.
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页数：16
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