Longan pulp polysaccharides relieve intestinal injury in vivo and in vitro by promoting tight junction expression

被引：88
作者：
Bai, Yajuan [1 ]
Huang, Fei [1 ]
Zhang, Ruifen [1 ]
Dong, Lihong [1 ]
Jia, Xuchao [1 ]
Liu, Lei [1 ]
Yi, Yang [2 ]
Zhang, Mingwei [1 ]
机构：
[1] Minist Agr & Rural Affairs, Guangdong Acad Agr Sci, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods,Sericultural & Agri Food Res, Guangzhou 510610, Guangdong, Peoples R China
[2] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2020年 / 229卷
基金：
中国国家自然科学基金;
关键词：
Polysaccharides; Longan pulp; Intestinal barrier; Tight junction; Structural characteristic; STRUCTURAL-CHARACTERIZATION; IMMUNOMODULATORY ACTIVITY; PHYSICOCHEMICAL PROPERTIES; ACAUDINA-MOLPADIOIDES; DIETARY FUCOIDAN; CYCLOPHOSPHAMIDE; ANTIOXIDANT; EXTRACTION; BARRIER; ANTITUMOR;
D O I：
10.1016/j.carbpol.2019.115475
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The integrity of the intestinal mucosal barrier is important for the health of the host. In this study, longan pulp polysaccharides (LP) prevented the intestinal mucosal injury by increasing the expression of mucin 2, tight junction proteins zonulae occludens-1 (ZO-1), claudin-1, claudin-4, and adherens junction E-cadherin in cyclophosphamide-treated mice. To further identify the principle bioactive component of LP, four acidic polysaccharides (LPIa, LPIIa, LPIIIa, and LPIVa) were purified, and their intestinal protection activity in vitro was compared. LPIa, LPIIa, and LPIIIa displayed an ability to increase mRNA expression of ZO-1, claudin-1, occludin, and E-cadherin in differentiated Caco-2 cells, especially LPIa. LPIa has specific structure characteristics: porous surface structure, a high molecular weight (1.47 x 105 Da), and two specific glycosidic linkages of a-Araf-(1 > and 5)-a-Araf-(1. These structure characteristics might primarily contribute to greater intestinal barrier protective effect of LPIa.
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页数：12
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