Process Optimization for the Extraction of Polyphenol from Malaysian Brown Seaweed, Sargassum sp.

The optimum extraction parameters of Malaysian brown seaweed, Sargassum sp. were determined by using Response Surface Methodology (RSM). Central Composite Face-centered Design (CCFD) was used to study the influence of extraction temperature (40-60 degrees C), extraction time (60-180 minute) and liquid to solid ratio (20-40 mL/g) on total phenolic content (TPC) of Sargassum sp. dried extracts. The statistical analysis of the experiment indicated that extraction temperatue and time had significant effect on Sargassum sp. total phenolic contents (TPC). The CCFD showed that quadratic regression models were in good agreement with the experimental results with the coefficients of determination (R-2) of 0.8066 for Sargassum sp. TPC. The extraction temperature of 40 degrees C, extraction time of 180 minute and liquid-solid ratio of 40 mL/g were the most efficient for the extraction of polyphenols from Sargassum sp. dried powder.