Flow cytometry and automatic blood cell analysis in striped bass Morone saxatilis (Walbaum, 1792): A new hematological approach

被引：58

作者：

Fazio, Francesco ^{[1
]}

Saoca, Concetta ^{[1
]}

Costa, Gregorio ^{[2
]}

Zumbo, Alessandro ^{[1
]}

Piccione, Giuseppe ^{[1
]}

Parrino, Vincenzo ^{[3
]}

机构：

[1] Univ Messina, Polo Univ Annunziata, Dept Vet Sci, I-98168 Messina, Italy

[2] Univ Messina, Dept Human Pathol Adult & Dev Age, Messina, Italy

[3] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy

来源：

AQUACULTURE | 2019年 / 513卷

关键词：

Automatic cell count; Blood analytic techniques; Blood cells; Flow cytometry; Morone saxatilis; SERUM-PROTEIN PROFILES; BREAM SPARUS-AURATA; MUGIL-CEPHALUS; DICENTRARCHUS-LABRAX; GOBIUS-NIGER; FARO LAKE; AQUACULTURE; PARAMETERS; LEUKOCYTES; CHRYSOPS;

D O I：

10.1016/j.aquaculture.2019.734398

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

This research was carried out in order to compare two innovative analytical methods for the evaluation of blood cells (white blood cells, WBC; red blood cells, RBC and thrombocytes, TC) in Striped bass Morone saxatilis (Walbaum, 1792). For this purpose, the automatic blood cell count and the flow cytometry analysis were employed to analyze blood samples of 20 adult specimens of M. saxatilis, bred in a recirculating aquaculture system (RAS) of a fish farm located in Acate (Ragusa, Sicily, Italy). Statistical analysis of the data has shown a significant positive correlation between the two analytical methods only for WBC (r = 0.95) and RBC (r = 0.97). No significant correlation was found for TC (r = - 0.18). This study aims to contribute to the knowledge on blood cell analysis with reliable automatic methods applicable in aquaculture which represent an important and useful tool for the diagnosis of fish diseases.

引用

页数：5

共 35 条

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