Progesterone-dependent and -independent expression of the multidrug resistance type I gene in porcine granulosa cells

被引：5
作者：
Fukuda, Hiroaki [1 ]
He, Pei Jian [1 ]
Yokota, Kazuko [1 ]
Soh, Tomoki [1 ]
Yamauchi, Nobuhiko [1 ]
Hattori, Masa-aki [1 ]
机构：
[1] Kyushu Univ, Grad Sch, Fac Agr,Lab Reprod Physiol & Biotechnol, Dept Anim & Marine Bioresource Sci,Higashi Ku, Fukuoka 8128581, Japan
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2007年 / 298卷 / 1-2期
关键词：
MDRI expression; progesterone; RU-486; granulosa cells; promoter activity;
D O I：
10.1007/s11010-006-9364-7
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A primary role of plasma membrane P-glycoprotein (P-gp), encoded by multidrug resistance type I (MDR1), is to protect against naturally occurring xenotoxics. Progesterone (P-4) profoundly influences MDR1 expression in granulosa cells and luteal cells. Here, P4 regulation of MDR1 expression was investigated in porcine granulosa cells using the P-4-mediated promoter activity assay and a P4 receptor (PR) antagonist (RU-486). The promoter activity was measured chronologically for 48 h in cells transfected with the PR response element-containing pGL3. LH could stimulate the promoter activity through endogenous P4, with a maximum activity at 5 h. MDR1 mRNA level was highly maintained at 24-36 h. Conversely, exogenous P4 prolonged the promoter activity to further 10 h, and the high level of MDR1 mRNA was maintained even at 48 h. RU-486 completely inhibited the promoter activity, but the level of MDR1 mRNA rapidly increased in the presence of RU-486. The granulosa cells may become susceptible to RU-486 as a xenotoxic to rapidly express MDR1 for protection against it. These results indicate that MDR1 is expressed in porcine granulosa cells through P4-dependent and -independent regulations.
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页码：179 / 186
页数：8
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