MiRNA-26b regulates the expression of cyclooxygenase-2 in desferrioxamine-treated CNE cells

被引：60

作者：

Ji, Yanhong ^{[1
,2
]}

He, Yonghong ^{[3
]}

Liu, Le ^{[3
]}

Zhong, Xingyuan ^{[3
]}

机构：

[1] S China Normal Univ, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[2] S China Normal Univ, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China

[3] Tsinghua Univ, Grad Sch Shenzhen, Div Life Sci, Shenzhen 518055, Peoples R China

来源：

FEBS LETTERS | 2010年 / 584卷 / 05期

关键词：

MiR-26b; Cyclooxygenase-2; Desferrioxamine; Carcinoma of nasopharygeal epithelia cells; ENDOTHELIAL GROWTH-FACTOR; COLON-CARCINOMA CELLS; PROSTATE-CANCER; GENE-EXPRESSION; NUCLEAR-FACTOR; UP-REGULATION; C/EBP-BETA; KAPPA-B; HYPOXIA; MICRORNAS;

D O I：

10.1016/j.febslet.2010.01.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report that miR-26b is involved in COX-2 overexpression in desferrioxamine (DFOM)treated carcinoma of nasopharyngeal epithelial (CNE) cells. The level of miR-26b in DFOM-treated CNE cells is inversely proportional to the expression level of the COX-2 protein. Overexpression of miR-26b in DFOM-treated CNE cells inhibits cell proliferation. A luciferase reporter gene experiment suggests that the 30 untranslated region of COX-2 carries a binding site for miR-26b. Overexpression of miR-26b marginally reduces the levels of COX-2 protein in DFOM-treated CNE cells. Moreover, knockdown of COX-2 expression had a similar effect to overexpression of miR-26b. Taken together, these results suggest that miR-26b regulates COX-2 expression in DFOM-treated cells. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：961 / 967

页数：7

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