A study was conducted to investigate response of male and female pistachio seedlings to salinity as factorial in framework completely randomized design. Treatments were contained salinity at levels of 0, 60 and 120 mM and sexuality of seedlings at two levels of male and female. According to results, under salinity stress, the number of green leaves, shoots dry weight and stem height had less decreasing in male seedlings than female. Based on measured parameters in present study was identified that at the salinity level of 60 mM, male seedlings had more resistance than female, but at the salinity level of 120 mM, there was not a significant difference between the male and female seedlings. (C) 2016 Elsevier B.V. All rights reserved.