Biofabrication of Collagen Tissue-Engineered Blood Vessels with Direct Co-Axial Extrusion

被引:13
作者
Bosch-Rue, Elia [1 ,2 ]
Diez-Tercero, Leire [1 ,2 ]
Delgado, Luis M. [1 ,2 ]
Perez, Roman A. [1 ,2 ]
机构
[1] Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
[2] Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, Spain
关键词
tissue-engineered blood vessels; collagen; co-axial extrusion; vascular construct; cell-laden hydrogels; SAPHENOUS-VEIN; PRESSURE;
D O I
10.3390/ijms23105618
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cardiovascular diseases are considered one of the worldwide causes of death, with atherosclerosis being the most predominant. Nowadays, the gold standard treatment is blood vessel replacement by bypass surgery; however, autologous source is not always possible. Thereby, tissue-engineered blood vessels (TEBVs) are emerging as a potential alternative source. In terms of composition, collagen has been selected in many occasions to develop TEBVs as it is one of the main extracellular matrix components of arteries. However, it requires specific support or additional processing to maintain the tubular structure and appropriate mechanical properties. Here, we present a method to develop support-free collagen TEBVs with co-axial extrusion in a one-step procedure with high concentrated collagen. The highest concentration of collagen of 20 mg/mL presented a burst pressure of 619.55 +/- 48.77 mmHg, being able to withstand perfusion of 10 dynes/cm(2). Viability results showed a high percentage of viability (86.1 and 85.8% with 10 and 20 mg/mL, respectively) of human aortic smooth muscle cells (HASMCs) and human umbilical vein endothelial cells (HUVEC) after 24 h extrusion. Additionally, HUVEC and HASMCs were mainly localized in their respective layers, mimicking the native distribution. All in all, this approach allows the direct extrusion of collagen TEBVs in a one-step procedure with enough mechanical properties to be perfused.
引用
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页数:12
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共 36 条
[1]   A model of guided cell self-organization for rapid and spontaneous formation of functional vessels [J].
Andrique, L. ;
Recher, G. ;
Alessandri, K. ;
Pujol, N. ;
Feyeux, M. ;
Bon, P. ;
Cognet, L. ;
Nassoy, P. ;
Bikfalvi, A. .
SCIENCE ADVANCES, 2019, 5 (06)
[2]  
Athanasiou KA, 2013, ANNU REV BIOMED ENG, V15, P115, DOI [10.1146/annurev-bioeng-071812-15242, 10.1146/annurev-bioeng-071812-152423]
[3]   3-D bioprinting technologies in tissue engineering and regenerative medicine: Current and future trends [J].
Bishop, Elliot S. ;
Mostafa, Sami ;
Pakvasa, Mikhail ;
Luu, Hue H. ;
Lee, Michael J. ;
Wolf, Jennifer Moriatis ;
Ameer, Guillermo A. ;
He, Tong-Chuan ;
Reid, Russell R. .
GENES & DISEASES, 2017, 4 (04) :185-195
[4]   Direct extrusion of individually encapsulated endothelial and smooth muscle cells mimicking blood vessel structures and vascular native cell alignment [J].
Bosch-Rue, E. ;
Delgado, Luis M. ;
Gil, F. Javier ;
Perez, Roman A. .
BIOFABRICATION, 2021, 13 (01)
[5]   The mechanical characterization of blood vessels and their substitutes in the continuous quest for physiological-relevant performances. A critical review [J].
Camasao, D. B. ;
Mantovani, D. .
MATERIALS TODAY BIO, 2021, 10
[6]   Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing [J].
Chang, Robert ;
Sun, Wei .
TISSUE ENGINEERING PART A, 2008, 14 (01) :41-48
[7]   Acetic acid and pepsin result in high yield, high purity and low macrophage response collagen for biomedical applications [J].
Delgado, Luis M. ;
Shologu, Naledi ;
Fuller, Kieran ;
Zeugolis, Dimitrios I. .
BIOMEDICAL MATERIALS, 2017, 12 (06)
[8]   Human Vascular Microphysiological System for in vitro Drug Screening [J].
Fernandez, C. E. ;
Yen, R. W. ;
Perez, S. M. ;
Bedell, H. W. ;
Povsic, T. J. ;
Reichert, W. M. ;
Truskey, G. A. .
SCIENTIFIC REPORTS, 2016, 6
[9]   Off-pump coronary artery bypass grafting using a bilateral internal mammary artery Y graft [J].
Gu, Cheng-Xiong ;
Yang, Jun-Feng ;
Zhang, Hong-Chao ;
Wei, Hua ;
Li, Ling-Ke .
JOURNAL OF GERIATRIC CARDIOLOGY, 2012, 9 (03) :247-251
[10]   Contractile responses in arteries subjected to hypertensive pressure in seven-day organ culture [J].
Han, HC ;
Ku, DN .
ANNALS OF BIOMEDICAL ENGINEERING, 2001, 29 (06) :467-475