Biofabrication of Collagen Tissue-Engineered Blood Vessels with Direct Co-Axial Extrusion
被引:13
作者:
Bosch-Rue, Elia
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h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Bosch-Rue, Elia
[1
,2
]
Diez-Tercero, Leire
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Diez-Tercero, Leire
[1
,2
]
Delgado, Luis M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Delgado, Luis M.
[1
,2
]
Perez, Roman A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
Perez, Roman A.
[1
,2
]
机构:
[1] Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Barcelona 08195, Spain
[2] Univ Int Catalunya UIC, Basic Sci Dept, Barcelona 08195, Spain
Cardiovascular diseases are considered one of the worldwide causes of death, with atherosclerosis being the most predominant. Nowadays, the gold standard treatment is blood vessel replacement by bypass surgery; however, autologous source is not always possible. Thereby, tissue-engineered blood vessels (TEBVs) are emerging as a potential alternative source. In terms of composition, collagen has been selected in many occasions to develop TEBVs as it is one of the main extracellular matrix components of arteries. However, it requires specific support or additional processing to maintain the tubular structure and appropriate mechanical properties. Here, we present a method to develop support-free collagen TEBVs with co-axial extrusion in a one-step procedure with high concentrated collagen. The highest concentration of collagen of 20 mg/mL presented a burst pressure of 619.55 +/- 48.77 mmHg, being able to withstand perfusion of 10 dynes/cm(2). Viability results showed a high percentage of viability (86.1 and 85.8% with 10 and 20 mg/mL, respectively) of human aortic smooth muscle cells (HASMCs) and human umbilical vein endothelial cells (HUVEC) after 24 h extrusion. Additionally, HUVEC and HASMCs were mainly localized in their respective layers, mimicking the native distribution. All in all, this approach allows the direct extrusion of collagen TEBVs in a one-step procedure with enough mechanical properties to be perfused.
机构:
INSERM, U1029, LAMC, F-33170 Pessac, France
Univ Bordeaux, F-33170 Pessac, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Andrique, L.
;
Recher, G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Recher, G.
;
Alessandri, K.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Alessandri, K.
;
Pujol, N.
论文数: 0引用数: 0
h-index: 0
机构:
INSERM, U1029, LAMC, F-33170 Pessac, France
Univ Bordeaux, F-33170 Pessac, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Pujol, N.
;
Feyeux, M.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Feyeux, M.
;
Bon, P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Bon, P.
;
Cognet, L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Cognet, L.
;
Nassoy, P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Bosch-Rue, E.
;
Delgado, Luis M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Delgado, Luis M.
;
Gil, F. Javier
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Gil, F. Javier
;
Perez, Roman A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Gu, Cheng-Xiong
;
Yang, Jun-Feng
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Yang, Jun-Feng
;
Zhang, Hong-Chao
论文数: 0引用数: 0
h-index: 0
机构:
Airfore Gen Hosp, Dept Cardiovasc Surg, Beijing 100142, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Zhang, Hong-Chao
;
Wei, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Wei, Hua
;
Li, Ling-Ke
论文数: 0引用数: 0
h-index: 0
机构:
Airfore Gen Hosp, Dept Cardiovasc Surg, Beijing 100142, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
机构:
INSERM, U1029, LAMC, F-33170 Pessac, France
Univ Bordeaux, F-33170 Pessac, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Andrique, L.
;
Recher, G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Recher, G.
;
Alessandri, K.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Alessandri, K.
;
Pujol, N.
论文数: 0引用数: 0
h-index: 0
机构:
INSERM, U1029, LAMC, F-33170 Pessac, France
Univ Bordeaux, F-33170 Pessac, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Pujol, N.
;
Feyeux, M.
论文数: 0引用数: 0
h-index: 0
机构:
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Feyeux, M.
;
Bon, P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Bon, P.
;
Cognet, L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
Cognet, L.
;
Nassoy, P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bordeaux, Lab Photon Numer & Nanosci, LP2N, F-33400 Talence, France
Inst Opt, Grad Sch, F-33400 Talence, France
CNRS, UMR 5298, F-33400 Talence, FranceINSERM, U1029, LAMC, F-33170 Pessac, France
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Bosch-Rue, E.
;
Delgado, Luis M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Delgado, Luis M.
;
Gil, F. Javier
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
Gil, F. Javier
;
Perez, Roman A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, SpainUniv Int Catalunya UIC, Bioengn Inst Technol BIT, Sant Cugat Del Valles, Spain
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Gu, Cheng-Xiong
;
Yang, Jun-Feng
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Yang, Jun-Feng
;
Zhang, Hong-Chao
论文数: 0引用数: 0
h-index: 0
机构:
Airfore Gen Hosp, Dept Cardiovasc Surg, Beijing 100142, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Zhang, Hong-Chao
;
Wei, Hua
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China
Wei, Hua
;
Li, Ling-Ke
论文数: 0引用数: 0
h-index: 0
机构:
Airfore Gen Hosp, Dept Cardiovasc Surg, Beijing 100142, Peoples R ChinaCapital Med Univ, Beijing Anzhen Hosp, Dept Cardiac Surg, Beijing 100029, Peoples R China