Absence of the aflatoxin biosynthesis gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures

被引：13

作者：

Ehrlich, Kenneth C. ^{[1
]}

Chang, Perng-Kuang ^{[1
]}

Scharfenstein, Leslie L., Jr. ^{[1
]}

Cary, Jeffrey W. ^{[1
]}

Crawford, Jason M. ^{[2
]}

Townsend, Craig A. ^{[2
]}

机构：

[1] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA

[2] Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2010年 / 305卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Aspergillus flavus; aflatoxin biosynthesis; gene disruption; MS; aryl alcohol dehydrogenase; aflatoxicol; O-METHYLSTERIGMATOCYSTIN; CLUSTER; PARASITICUS; CYTOCHROME-P-450; STERIGMATOCYSTIN; IDENTIFICATION; PRECURSOR; PATHWAY; G(1);

D O I：

10.1111/j.1574-6968.2010.01914.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Biosynthesis of the highly toxic and carcinogenic aflatoxins in select Aspergillus species from the common intermediate O-methylsterigmatocystin has been postulated to require only the cytochrome P450 monooxygenase, OrdA (AflQ). We now provide evidence that the aryl alcohol dehydrogenase NorA (AflE) encoded by the aflatoxin biosynthetic gene cluster in Aspergillus flavus affects the accumulation of aflatoxins in the final steps of aflatoxin biosynthesis. Mutants with inactive norA produced reduced quantities of aflatoxin B-1 (AFB(1)), but elevated quantities of a new metabolite, deoxyAFB(1). To explain this result, we suggest that, in the absence of NorA, the AFB(1) reduction product, aflatoxicol, is produced and is readily dehydrated to deoxyAFB(1) in the acidic medium, enabling us to observe this otherwise minor toxin produced in wild-type A. flavus.

引用

页码：65 / 70

页数：6

共 22 条

[1] IDENTIFICATION OF O-METHYLSTERIGMATOCYSTIN AS AN AFLATOXIN-B1 AND AFLATOXIN-G1 PRECURSOR IN ASPERGILLUS-PARASITICUS [J].

BHATNAGAR, D ;

MCCORMICK, SP ;

LEE, LS ;

HILL, RA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (05) :1028-1033

[2] Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans [J].

Brown, DW ;

Yu, JH ;

Kelkar, HS ;

Fernandes, M ;

Nesbitt, TC ;

Keller, NP ;

Adams, TH ;

Leonard, TJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) :1418-1422

[3] Molecular characterization of an Aspergillus parasiticus dehydrogenase gene, norA, located on the aflatoxin biosynthesis gene cluster [J].

Cary, JW ;

Wright, M ;

Bhatnagar, D ;

Lee, R ;

Chu, FS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :360-366

[4] Characterization of the Aspergillus parasiticus niaD and niiA gene cluster [J].

Chang, PK ;

Ehrlich, KC ;

Linz, JE ;

Bhatnagar, D ;

Cleveland, TE ;

Bennett, JW .

CURRENT GENETICS, 1996, 30 (01) :68-75

[5] EVIDENCE FOR THE PROBABLE FINAL STEPS IN AFLATOXIN BIOSYNTHESIS [J].

CHATTERJEE, M ;

TOWNSEND, CA .

JOURNAL OF ORGANIC CHEMISTRY, 1994, 59 (16) :4424-4429

[6] Cloning of a genetically unstable cytochrome P-450 gene cluster involved in degradation of the pollutant ethyl tert-butyl ether by Rhodococcus ruber [J].

Chauvaux, S ;

Chevalier, F ;

Le Dantec, C ;

Fayolle, F ;

Miras, I ;

Kunst, F ;

Beguin, P .

JOURNAL OF BACTERIOLOGY, 2001, 183 (22) :6551-6557

[7] Aflatoxin biosynthesis gene clusters and flanking regions [J].

Ehrlich, KC ;

Yu, J ;

Cotty, PJ .

JOURNAL OF APPLIED MICROBIOLOGY, 2005, 99 (03) :518-527

[8] Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation [J].

Ehrlich, KC ;

Chang, PK ;

Yu, JJ ;

Cotty, PJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (11) :6518-6524

[9] Are the genes nadA and norB involved in formation of aflatoxin G1? [J].

Ehrlich, Kenneth C. ;

Scharfenstein, Leslie L., Jr. ;

Montalbano, Beverly G. ;

Chang, Perng-Kuang .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2008, 9 (09) :1717-1729

[10] Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins [J].

Frisvad, J. C. ;

Larsen, T. O. ;

de Vries, R. ;

Meijer, M. ;

Houbraken, J. ;

Cabanes, F. J. ;

Ehrlich, K. ;

Samson, R. A. .

STUDIES IN MYCOLOGY, 2007, (59) :31-37

← 1 2 3 →