Effect of inoculation with ecto- and arbuscular mycorrhizae and Rhizobium on the growth and nitrogen fixation by black locust, Robinia pseudoacacia

Experiments were made to determine the effect of inoculation with Rhizobium, the ectomycorrhizal fungus Hebeloma mesophasem and the arbuscular mycorrhizal fungus Glomus caledonium on the growth and nitrogen fixation of black locust (Robinia pseudoacacia Linn.) seedlings grown in vitro and in pot culture. The results showed that both mycorrhizal fungi and Rhizobium stimulated the growth and nitrogen fixing ability of inoculated seedlings. Inoculation with all three microbes together produced the most beneficial effects on nitrogen fixation, mycorrhizal development and seedling growth.