Purification and characterization of a novel biosurfactant produced by Bacillus licheniformis MS3

被引：28

作者：

Biria, D. ^{[1
]}

Maghsoudi, E. ^{[2
]}

Roostaazad, R. ^{[1
]}

Dadafarin, H. ^{[3
]}

Lotfi, A. Sahebghadam ^{[4
]}

Amoozegar, M. A. ^{[5
]}

机构：

[1] Sharif Univ Technol, Dept Chem & Petr Engn, Tehran, Iran

[2] Ecole Polytech Univ, Dept Civil Engn, Montreal, PQ, Canada

[3] McGill Univ, Dept Chem Engn, Montreal, PQ, Canada

[4] Ctr Genet Engn & Biotechnol, Tehran, Iran

[5] Univ Tehran, Dept Biol, Fac Sci, Tehran, Iran

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2010年 / 26卷 / 05期

关键词：

Biosurfactant; Bacillus licheniformis; Lipopeptide; Surface tension; Emulsification; LIPOPEPTIDE;

D O I：

10.1007/s11274-009-0246-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The physical properties and chemical structure of a new biosurfactant (licheniformin) produced by Bacillus licheniformis MS3 were investigated. The purified biosurfactant was identified as a lipopeptide with amino acid sequence of Gly, Ala, Val, Asp, Ser, Gly, Tyr and a lactone linkage between the carboxyl group of Aspargine and hydroxyl group of Tyrosine residue. The fatty acid moiety was attached to N-terminal amino acid residue through an amide bond. The purified licheniformin could lower the surface tension of water from 72 to 38 mN/m at concentrations higher than 15 mu g/mL and its relative emulsion volume (EV%) was equal to 36%. It also showed stable surface activity over a wide range of temperature (45-85A degrees C) and pH (3-11).

引用

页码：871 / 878

页数：8

共 24 条

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