Development of EST-derived microsatellite markers for Arabidopsis lyrata subspecies petraea (L.)

被引：5
|
作者：
Woodhead, M. [1 ]
Russell, J.
Squirrell, J.
Hollingsworth, P. M.
Cardle, L.
Gibby, M.
Powell, W.
机构：
[1] Scottish Crop Res Inst, Dundee DD2 5DA, Scotland
[2] Royal Bot Gardens, Edinburgh EH3 5LR, Midlothian, Scotland
[3] NIAB, Cambridge CB3 OLE, England
来源：
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 04期
关键词：
cDNA; EST; microsatellite; Arabidopsis lyrata;
D O I：
10.1111/j.1471-8286.2006.01656.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A set of expressed sequence tag-simple sequence repeat (EST-SSR) loci has been developed for Arabidopsis lyrata ssp. petraea. From 768 root cDNA clones, 126 microsatellites, including di-, tri-, tetra- and pentanucleotide repeat motifs were identified and primers were designed to 24 EST-SSRs. Eleven loci were subsequently screened on 150 individuals sampled from five natural populations, which revealed three to nine alleles per locus (mean 5.36) and expected heterozygosity (H-E) estimates ranging from 0.046 to 0.698. Significant deviations from random mating were observed at 10 EST-SSR loci, likely due to inbreeding (global F-IS = 0.151) and population structure (global F-ST = 0.246).
引用
收藏
页码：631 / 634
页数：4
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