Immunohistochemical detection of virulence-associated Rhodococcus equi antigens in pulmonary and intestinal lesions in horses

被引：9
作者：
Mariotti, F
Cuteri, V
Takai, S
Renzoni, G
Pascucci, L
Vitellozzi, G
机构：
[1] Univ Perugia, Fac Med Vet, Dipartimento Sci Biopatol, I-06126 Perugia, Italy
[2] Univ Camerino, Fac Med Vet, Dipartimento Sci Vet, I-62032 Camerino, Italy
[3] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Anim Hyg, Tawada, Aomori, Japan
[4] Univ Perugia, Fac Med Vet, Dipartimento Sci Biopatol, I-06126 Perugia, Italy
来源：
JOURNAL OF COMPARATIVE PATHOLOGY | 2000年 / 123卷 / 2-3期
关键词：
D O I：
10.1053/jcpa.2000.0392
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Two horses with Rhodococcus equi infection were examined post mortem by an immunohistochemical method (peroxidase-antiperoxidase; PAP) with a monoclonal antibody (Mab 10G5) to the 15-17 kDa antigen of R. equi. One of the horses was also examined bacteriologically, R. equi being isolated in culture. Immunolabelling with this Mab was marked and widespread. On the other hand, the immunohistochemical reactivity of infected macrophages with a polyclonal antibody specific for lysozyme was slight. Thus, Mab 10G5 would appear to be a useful diagnostic reagent in R. equi infection, with or without cultural confirmation. (C) 2000 Harcourt Publishers Ltd.
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页码：186 / 189
页数：4
相关论文
共 8 条
[1]   RHODOCOCCUS-EQUI INFECTION IN PATIENTS WITH AIDS [J].
DRANCOURT, M ;
BONNET, E ;
GALLAIS, H ;
PELOUX, Y ;
RAOULT, D .
JOURNAL OF INFECTION, 1992, 24 (02) :123-131
[2]  
JUBB KVF, 1993, PATHOLOGY DOMESTIC A, V2, P652
[3]   Immunohistochemical detection of virulence-associated antigens of Rhodococcus equi in pulmonary lesions of foals [J].
Madarame, H ;
Takai, S ;
Morisawa, N ;
Fujii, M ;
Hidaka, D ;
Tsubaki, S ;
Hasegawa, Y .
VETERINARY PATHOLOGY, 1996, 33 (03) :341-343
[4]   EVALUATION OF A MONOCLONAL ANTIBODY-BASED COLONY BLOT TEST FOR RAPID IDENTIFICATION OF VIRULENT RHODOCOCCUS-EQUI [J].
TAKAI, S ;
MORISHITA, T ;
NISHIO, Y ;
SASAKI, Y ;
TSUBAKI, S ;
HIGUCHI, T ;
HAGIWARA, S ;
SENBA, H ;
KATO, M ;
SENO, N ;
ANZAI, T ;
KAMADA, M .
JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (04) :681-684
[5]   Expression of virulence-associated antigens of Rhodococcus equi is regulated by temperature and pH [J].
Takai, S ;
Fukunaga, N ;
Kamisawa, K ;
Imai, Y ;
Sasaki, Y ;
Tsubaki, S .
MICROBIOLOGY AND IMMUNOLOGY, 1996, 40 (08) :591-594
[6]   IDENTIFICATION OF 15-KILODALTON TO 17-KILODALTON ANTIGENS ASSOCIATED WITH VIRULENT RHODOCOCCUS-EQUI [J].
TAKAI, S ;
KOIKE, K ;
OHBUSHI, S ;
IZUMI, C ;
TSUBAKI, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) :439-443
[7]   VIRULENCE-ASSOCIATED PLASMIDS IN RHODOCOCCUS-EQUI [J].
TAKAI, S ;
WATANABE, Y ;
IKEDA, T ;
OZAWA, T ;
MATSUKURA, S ;
TAMADA, Y ;
TSUBAKI, S ;
SEKIZAKI, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (07) :1726-1729
[8]  
Takai Shinji, 1995, Journal of Equine Science, V6, P105, DOI 10.1294/jes.6.105
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