Structure-guided stereoselectivity inversion of a short-chain dehydrogenase/reductase towards halogenated acetophenones

被引：40

作者：

Li, Aipeng ^{[1
]}

Ye, Lidan ^{[1
,2
]}

Yang, Xiaohong ^{[1
]}

Yang, Chengcheng ^{[1
]}

Gu, Jiali ^{[3
]}

Yu, Hongwei ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Chem & Biol Engn, Inst Bioengn, Hangzhou 310027, Zhejiang, Peoples R China

[2] Zhejiang Univ, Minist Educ, Key Lab Biomass Chem Engn, Hangzhou 310027, Zhejiang, Peoples R China

[3] Huzhou Univ, Dept Chem Mat, Coll Life Sci, Huzhou 313000, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 37期

关键词：

SECONDARY ALCOHOL-DEHYDROGENASE; CARBONYL REDUCTASE; ENANTIOSELECTIVITY; PRELOG; MUTATION; REDUCTIONS; ESTERASE;

D O I：

10.1039/c6cc00051g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The structure-guided rational design of an NADH-dependent short-chain dehydrogenase/reductase (SDR) reversed the stereoselectivity towards halogenated acetophenones from Prelog to anti-Prelog. The enzyme-substrate interactions involving an aromatic ring and a halogen atom were proven to play critical roles in determining the stereoselectivity of these ketone reductions besides the steric repulsion.

引用

页码：6284 / 6287

页数：4

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