RETRACTED: RETRACTED:Inhibition of KRAS gene mutation on non-small cell lung cancer and its effect on circulating tumor cells (Retracted article. See vol. 111, pg. 937, 2020)

Purpose: discuss effect of miR-200C on proliferation in vitro of non-small-cell ovarian carcinoma cells and mechanism. Method: use lentivirus infection method to establish miR-200c non-small-cell ovarian carcinoma cells of stable over-expression and stable low expression which is divided into control group, stable over-expression miR-200c group (miR-200c group) and stable low stable expression miR-200c group. Make real-time quantification for qRT-PCR and Western blot, detect expression quantity of miR-200c and KRAS in non-small-cell ovarian carcinoma cells of different experimental groups, and detect proliferation ability of non-small-cell ovarian carcinoma cell line in different groups by clone test. Result: CCK-8 test shows that control test T24, UM-UC-3 and miR-200c group is 0.8333 +/- 0.0621, 0.6503 +/- 0.0887 and 0.9390 +/- 0.0181, 0.8060 +/- 0.0216 respectively. Difference between 2 groups has statistical significance (T24, P < 0.05; UM-UC-3, P < 0.01). After stable over-expression miR-200c, expression of KRAS protein in non-small-cell ovarian carcinoma cells is reduced (P < 0.05). Conclusion: miR-200c reduces proliferation ability of non-small-cell ovarian carcinoma cells by regulating KRAS gene down. (C) 2018 Elsevier B.V. All rights reserved.